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Open AccessArticle

Development of an Electrochemical Sensor for NADH Determination Based on a Caffeic Acid Redox Mediator Supported on Carbon Black

1
Department of Chemical and Geological Sciences, University of Modena and Reggio Emilia, via G. Campi, 41125 Modena, Italy
2
Department of Chemical Sciences and Technologies, University of Tor Vergata, via della Ricerca Scientifica, 00133 Roma, Italy
3
Institute of Organic Synthesis and Photoreactivity (ISOF)—National Research Council of Italy (CNR), via Gobetti 101, 40129 Bologna, Italy
*
Author to whom correspondence should be addressed.
Academic Editors: Paolo Ugo and Michael Ongaro
Chemosensors 2015, 3(2), 118-128; https://doi.org/10.3390/chemosensors3020118
Received: 27 February 2015 / Revised: 27 March 2015 / Accepted: 1 April 2015 / Published: 13 April 2015
(This article belongs to the Special Issue Electrochemical Sensors for Environmental and Food Analysis)
Screen-printed electrode (SPE) modified with carbon black nanoparticles (CB) has been tested as a new platform for the stable deposition of caffeic acid (CFA) on the electrode surface. The electrochemical performance from varying the amount of CFA/CB composite has been tested with respect to NADH determination. The electrocatalytic activity of CFA/CB has also been compared with that of SPEs modified by a single component of the coating, i.e., either CFA or CB. Finally, glycerol dehydrogenase, a typical NADH-dependent enzyme, was deposited on the CFA/CB coating in order to test the applicability of the sensor in glycerol determination. View Full-Text
Keywords: screen-printed electrodes; amperometric sensor; enzymatic biosensors; NADH; caffeic acid; carbon black; glycerol dehydrogenase; glycerol determination screen-printed electrodes; amperometric sensor; enzymatic biosensors; NADH; caffeic acid; carbon black; glycerol dehydrogenase; glycerol determination
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MDPI and ACS Style

Zanardi, C.; Ferrari, E.; Pigani, L.; Arduini, F.; Seeber, R. Development of an Electrochemical Sensor for NADH Determination Based on a Caffeic Acid Redox Mediator Supported on Carbon Black. Chemosensors 2015, 3, 118-128.

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