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Loop Mediated Isothermal Amplification: Principles and Applications in Plant Virology

1
Department of Agricultural, Food and Forest Sciences, University of Palermo, 90128 Palermo, Italy
2
Department of Agricultural, Forestry and Food Sciences, University of Turin, 10095 Turin, Italy
3
Council for Agricultural Research and Economics, Research Center for Plant Protection and Certification, 00156 Rome, Italy
4
Institute for Sustainable Plant Protection, National Research Council (IPSP-CNR), 10135 Turin, Italy
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Plants 2020, 9(4), 461; https://doi.org/10.3390/plants9040461
Received: 6 March 2020 / Revised: 2 April 2020 / Accepted: 2 April 2020 / Published: 6 April 2020
(This article belongs to the Special Issue Diagnosis and Control of Plant Viral Diseases)
In the last decades, the evolution of molecular diagnosis methods has generated different advanced tools, like loop-mediated isothermal amplification (LAMP). Currently, it is a well-established technique, applied in different fields, such as the medicine, agriculture, and food industries, owing to its simplicity, specificity, rapidity, and low-cost efforts. LAMP is a nucleic acid amplification under isothermal conditions, which is highly compatible with point-of-care (POC) analysis and has the potential to improve the diagnosis in plant protection. The great advantages of LAMP have led to several upgrades in order to implement the technique. In this review, the authors provide an overview reporting in detail the different LAMP steps, focusing on designing and main characteristics of the primer set, different methods of result visualization, evolution and different application fields, reporting in detail LAMP application in plant virology, and the main advantages of the use of this technique. View Full-Text
Keywords: loop-mediated isothermal amplification; LAMP; Bst DNA polymerase; primers; real-time monitoring; virus; viroids; plant virology loop-mediated isothermal amplification; LAMP; Bst DNA polymerase; primers; real-time monitoring; virus; viroids; plant virology
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MDPI and ACS Style

Panno, S.; Matić, S.; Tiberini, A.; Caruso, A.G.; Bella, P.; Torta, L.; Stassi, R.; Davino, S. Loop Mediated Isothermal Amplification: Principles and Applications in Plant Virology. Plants 2020, 9, 461.

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