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Article

Development and Characterization of an Ethyl Methane Sulfonate (EMS) Induced Mutant Population in Capsicum annuum L.

1
Department of Plant Science and Plant Genomics and Breeding Institute, Seoul National University, Seoul 08826, Korea
2
Radiation Breeding Team, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup 56212, Korea
*
Author to whom correspondence should be addressed.
Plants 2020, 9(3), 396; https://doi.org/10.3390/plants9030396
Received: 22 February 2020 / Revised: 18 March 2020 / Accepted: 19 March 2020 / Published: 23 March 2020
(This article belongs to the Special Issue Plant Mutation Breeding)
Plant breeding explores genetic diversity in useful traits to develop new, high-yielding, and improved cultivars. Ethyl methane sulfonate (EMS) is a chemical widely used to induce mutations at loci that regulate economically essential traits. Additionally, it can knock out genes, facilitating efforts to elucidate gene functions through the analysis of mutant phenotypes. Here, we developed a mutant population using the small and pungent ornamental Capsicum annuum pepper “Micro-Pep”. This accession is particularly suitable for mutation studies and molecular research due to its compact growth habit and small size. We treated 9500 seeds with 1.3% EMS and harvested 3996 M2 lines. We then selected 1300 (32.5%) independent M2 families and evaluated their phenotypes over four years. The mutants displayed phenotypic variations in plant growth, habit, leaf color and shape, and flower and fruit morphology. An experiment to optimize Targeting Induced Local Lesions IN Genomes (TILLING) in pepper detected nine EMS-induced mutations in the eIF4E gene. The M2 families developed here exhibited broad phenotypic variation and should be valuable genetic resources for functional gene analysis in pepper molecular breeding programs using reverse genetics tools, including TILLING. View Full-Text
Keywords: EMS; pepper; mutation breeding; TILLING; phenotyping EMS; pepper; mutation breeding; TILLING; phenotyping
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MDPI and ACS Style

Siddique, M.I.; Back, S.; Lee, J.-H.; Jo, J.; Jang, S.; Han, K.; Venkatesh, J.; Kwon, J.-K.; Jo, Y.D.; Kang, B.-C. Development and Characterization of an Ethyl Methane Sulfonate (EMS) Induced Mutant Population in Capsicum annuum L. Plants 2020, 9, 396. https://doi.org/10.3390/plants9030396

AMA Style

Siddique MI, Back S, Lee J-H, Jo J, Jang S, Han K, Venkatesh J, Kwon J-K, Jo YD, Kang B-C. Development and Characterization of an Ethyl Methane Sulfonate (EMS) Induced Mutant Population in Capsicum annuum L. Plants. 2020; 9(3):396. https://doi.org/10.3390/plants9030396

Chicago/Turabian Style

Siddique, Muhammad I.; Back, Seungki; Lee, Joung-Ho; Jo, Jinkwan; Jang, Siyoung; Han, Koeun; Venkatesh, Jelli; Kwon, Jin-Kyung; Jo, Yeong D.; Kang, Byoung-Cheorl. 2020. "Development and Characterization of an Ethyl Methane Sulfonate (EMS) Induced Mutant Population in Capsicum annuum L." Plants 9, no. 3: 396. https://doi.org/10.3390/plants9030396

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