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Open AccessArticle

Genome-Wide Analysis and Expression Profiling of Rice Hybrid Proline-Rich Proteins in Response to Biotic and Abiotic Stresses, and Hormone Treatment

1
Department of Biotechnology, Panjab University, Chandigarh 160014, Punjab, India
2
National Agri-Food Biotechnology Institute, Mohali 140306, Punjab, India
3
Department of Microbiology, Panjab University, Chandigarh 160014, Punjab, India
4
National Institute of Plant Biotechnology, New Delhi 110012, India
*
Authors to whom correspondence should be addressed.
Plants 2019, 8(9), 343; https://doi.org/10.3390/plants8090343
Received: 29 June 2019 / Revised: 19 August 2019 / Accepted: 22 August 2019 / Published: 11 September 2019
(This article belongs to the Section Plant Genetics and Genomics)
Hybrid proline-rich proteins (HyPRPs) belong to the family of 8-cysteine motif (8CM) containing proteins that play important roles in plant development processes, and tolerance to biotic and abiotic stresses. To gain insight into the rice HyPRPs, we performed a systematic genome-wide analysis and identified 45 OsHyPRP genes encoding 46 OsHyPRP proteins. The phylogenetic relationships of OsHyPRP proteins with monocots (maize, sorghum, and Brachypodium) and a dicot (Arabidopsis) showed clustering of the majority of OsHyPRPs along with those from other monocots, which suggests lineage-specific evolution of monocots HyPRPs. Based on our previous RNA-Seq study, we selected differentially expressed OsHyPRPs genes and used quantitative real-time-PCR (qRT-PCR) to measure their transcriptional responses to biotic (Magnaporthe oryzae) and abiotic (heat, cold, and salt) stresses and hormone treatment (Abscisic acid; ABA, Methyl-Jasmonate; MeJA, and Salicylic acid; SA) in rice blast susceptible Pusa Basmati-1 (PB1) and blast-resistant near-isogenic line PB1+Pi9. The induction of OsHyPRP16 expression in response to the majority of stresses and hormonal treatments was highly correlated with the number of cis-regulatory elements present in its promoter region. In silico docking analysis of OsHyPRP16 showed its interaction with sterols of fungal/protozoan origin. The characterization of the OsHyPRP gene family enables us to recognize the plausible role of OsHyPRP16 in stress tolerance. View Full-Text
Keywords: rice; hybrid proline-rich proteins; biotic stress; abiotic stress; Magnaporthe oryzae rice; hybrid proline-rich proteins; biotic stress; abiotic stress; Magnaporthe oryzae
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Kapoor, R.; Kumar, G.; Arya, P.; Jaswal, R.; Jain, P.; Singh, K.; Sharma, T.R. Genome-Wide Analysis and Expression Profiling of Rice Hybrid Proline-Rich Proteins in Response to Biotic and Abiotic Stresses, and Hormone Treatment. Plants 2019, 8, 343.

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