Next Article in Journal
Genome-Wide Analysis and Expression Profiling of Rice Hybrid Proline-Rich Proteins in Response to Biotic and Abiotic Stresses, and Hormone Treatment
Previous Article in Journal
Current Status and Future Prospects in Herbicide Discovery
Open AccessArticle

Molecular Cloning and Characterization of SQUAMOSA-Promoter Binding Protein-Like Gene FvSPL10 from Woodland Strawberry (Fragaria vesca)

1
State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China
2
Department of Plant Science, University of Tennessee, Knoxville, TN 37996, USA
*
Authors to whom correspondence should be addressed.
Plants 2019, 8(9), 342; https://doi.org/10.3390/plants8090342
Received: 14 July 2019 / Revised: 29 August 2019 / Accepted: 3 September 2019 / Published: 11 September 2019
(This article belongs to the Section Molecular Botany)
SQUAMOSA-promoter binding protein-like (SPL) proteins are plant-specific transcript factors that play essential roles in plant growth and development. Although many SPL genes have been well characterized in model plants like Arabidopsis, rice and tomato, the functions of SPLs in strawberry are still largely elusive. In the present study, we cloned and characterized FvSPL10, the ortholog of AtSPL9, from woodland strawberry. Subcellular localization shows FvSPL10 localizes in the cell nucleus. The luciferase system assay indicates FvSPL10 is a transcriptional activator, and both in vitro and in vivo assays indicate FvSPL10 could bind to the promoter of FvAP1 and activate its expression. Ectopic expression of FvSPL10 in Arabidopsis promotes early flowering and increases organs size. These results demonstrate the multiple regulatory roles of FvSPL10 in plant growth and development and lay a foundation for investigating the biological functions of FvSPL10 in strawberry. View Full-Text
Keywords: molecular cloning; characterization; FvSPL10; woodland strawberry molecular cloning; characterization; FvSPL10; woodland strawberry
Show Figures

Figure 1

MDPI and ACS Style

Xiong, J.; Bai, Y.; Ma, C.; Zhu, H.; Zheng, D.; Cheng, Z. Molecular Cloning and Characterization of SQUAMOSA-Promoter Binding Protein-Like Gene FvSPL10 from Woodland Strawberry (Fragaria vesca). Plants 2019, 8, 342.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map

1
Back to TopTop