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lpa1-5525: A New lpa1 Mutant Isolated in a Mutagenized Population by a Novel Non-Disrupting Screening Method

1
Department of Agricultural and Environmental Sciences-Production, Landscape, Agroenergy-Università degli Studi di Milano, Via Celoria 2, 20133 Milan, Italy
2
Department of Biosciences-Università degli Studi di Milano, Via Celoria 26, 20133 Milan, Italy
*
Author to whom correspondence should be addressed.
Plants 2019, 8(7), 209; https://doi.org/10.3390/plants8070209
Received: 15 June 2019 / Revised: 29 June 2019 / Accepted: 4 July 2019 / Published: 6 July 2019
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Abstract

Phytic acid, or myo-inositol 1,2,3,4,5,6-hexakisphosphate, is the main storage form of phosphorus in plants. It is localized in seeds, deposited as mixed salts of mineral cations in protein storage vacuoles; during germination, it is hydrolyzed by phytases to make available P together with all the other cations needed for seed germination. When seeds are used as food or feed, phytic acid and the bound cations are poorly bioavailable for human and monogastric livestock due to their lack of phytase activity. Therefore, reducing the amount of phytic acid is one strategy in breeding programs aimed to improve the nutritional properties of major crops. In this work, we present data on the isolation of a new maize (Zea mays L.) low phytic acid 1 (lpa1) mutant allele obtained by transposon tagging mutagenesis with the Ac element. We describe the generation of the mutagenized population and the screening to isolate new lpa1 mutants. In particular, we developed a fast, cheap and non-disrupting screening method based on the different density of lpa1 seed compared to the wild type. This assay allowed the isolation of the lpa1-5525 mutant characterized by a new mutation in the lpa1 locus associated with a lower amount of phytic phosphorus in the seeds in comparison with the wild type. View Full-Text
Keywords: maize; low phytic acid; regional mutagenesis; Ac transposon; density assay; free phosphate; Chen’s assay; PCR based molecular marker maize; low phytic acid; regional mutagenesis; Ac transposon; density assay; free phosphate; Chen’s assay; PCR based molecular marker
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Borlini, G.; Rovera, C.; Landoni, M.; Cassani, E.; Pilu, R. lpa1-5525: A New lpa1 Mutant Isolated in a Mutagenized Population by a Novel Non-Disrupting Screening Method. Plants 2019, 8, 209.

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