Next Article in Journal
New Insights into Leaf Physiological Responses to Ozone for Use in Crop Modelling
Previous Article in Journal
Post-Translational Modification of Proteins Mediated by Nitro-Fatty Acids in Plants: Nitroalkylation
Article Menu
Issue 4 (April) cover image

Export Article

Open AccessArticle

Karyological Analysis and DNA Barcoding of Pompia Citron: A First Step toward the Identification of Its Relatives

1
Dipartimento di AGRARIA Research Unit SACEG, University of Sassari, 07100 Sassari, Italy
2
Laboratory of Genomics, Department of Agronomy Food Natural Resources Animals and Environment, University of Padova, 35020 Legnaro, Padova, Italy
3
Unite Mixte de Recherche Amelioration Genetique et Adaptation des Plantes (UMR Agap), Institut National de la Recherche Agronomique (INRA), F-20230 San Giuliano, France
*
Author to whom correspondence should be addressed.
Plants 2019, 8(4), 83; https://doi.org/10.3390/plants8040083
Received: 18 February 2019 / Revised: 24 March 2019 / Accepted: 28 March 2019 / Published: 31 March 2019
(This article belongs to the Special Issue Genomics for Plant Breeding)
  |  
PDF [3542 KB, uploaded 24 April 2019]
  |  

Abstract

Pompia is a citrus fruit endemic of Sardinia, Italy, with an essential oil profile showing outstanding anti-inflammatory and anti-microbic properties. Despite its remarkable pharmaceutical potential, little taxonomic and genetic information is available for this species. We applied flow cytometry and classical cytogenetic techniques to assess the DNA content and to reconstruct the karyotype of several Pompia accessions. Molecular data from plastid DNA barcoding and nuclear DNA sequencing were used to study the genetic distance between Pompia and other citrus species. Flow cytometric estimates of DNA content and somatic chromosome counts suggest that Pompia is a regular diploid Citrus species. DNA polymorphisms of nuclear and chloroplast markers allowed us to investigate the genetic relationships between Pompia accessions and other Citrus species. Based on DNA polymorphism data we propose that Pompia is a very recent interspecific hybrid generated by a cross between C. aurantium (as seed bearer) and C. medica (as pollen donor). Our findings pave the way for further and more specific investigations of local Pompia germplasm resources that may help the preservation and valorisation of this valuable citrus fruit tree. View Full-Text
Keywords: Karyotype; DNA content; ITS sequence; psbA-trnH sequence; trnL intron sequence Karyotype; DNA content; ITS sequence; psbA-trnH sequence; trnL intron sequence
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Supplementary material

SciFeed

Share & Cite This Article

MDPI and ACS Style

Viglietti, G.; Galla, G.; Porceddu, A.; Barcaccia, G.; Curk, F.; Luro, F.; Scarpa, G.M. Karyological Analysis and DNA Barcoding of Pompia Citron: A First Step toward the Identification of Its Relatives. Plants 2019, 8, 83.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Plants EISSN 2223-7747 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top