Differential Expression Proteins Contribute to Race-Specific Resistant Ability in Rice (Oryza sativa L.)
Shiwei Ma 1,†
, Shoukai Lin 2,†, Menglin Wang 1, Yang Zou 1, Huan Tao 1, Wei Liu 1, Lina Zhang 1, Kangjing Liang 1, Yufang Ai 1,* and Huaqin He 1,*
, Shoukai Lin 2,†, Menglin Wang 1, Yang Zou 1, Huan Tao 1, Wei Liu 1, Lina Zhang 1, Kangjing Liang 1, Yufang Ai 1,* and Huaqin He 1,*
1
College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China
2
Key laboratory of Loquat Germplasm Innovation and Utilization, Putian University, Fujian Province University, Putian 351100, China
*
Authors to whom correspondence should be addressed.
†
The first 2 authors should be regarded as joint First Authors.
Plants 2019, 8(2), 29; https://doi.org/10.3390/plants8020029
Received: 7 December 2018 / Revised: 17 January 2019 / Accepted: 18 January 2019 / Published: 23 January 2019
(This article belongs to the Special Issue Induced Resistance (IR) of Plants)
Abstract
Rice blast, caused by the fungus, Magnaporthe grisea (M. grisea), lead to the decrease of rice yields widely and destructively, threatening global food security. Although many resistant genes had been isolated and identified in various rice varieties, it is still not enough to clearly understand the mechanism of race-specific resistant ability in rice, especially on the protein level. In this research, proteomic methods were employed to analyze the differentially expressed proteins (DEPs) in susceptible rice variety CO39 and its two near isogenic lines (NILs), CN-4a and CN-4b, in response to the infection of two isolates with different pathogenicity, GUY11 and 81278ZB15. A total of 50 DEPs with more than 1.5-fold reproducible change were identified. At 24 and 48 hpi of GUY11, 32 and 16 proteins in CN-4b were up-regulated, among which 16 and five were paralleled with the expression of their corresponding RNAs. Moreover, 13 of 50 DEPs were reported to be induced by M. grisea in previous publications. Considering the phenotypes of the three tested rice varieties, we found that 21 and 23 up-regulated proteins were responsible for the rice resistant ability to the two different blast isolates, 81278ZB15 and GUY11, respectively. Two distinct branches corresponding to GUY11 and 81278ZB15 were observed in the expression and function of the module cluster of DEPs, illuminating that the DEPs could be responsible for race-specific resistant ability in rice. In other words, DEPs in rice are involved in different patterns and functional modules’ response to different pathogenic race infection, inducing race-specific resistant ability in rice. View Full-TextKeywords:
rice (Oryza sativa L.); blast fungus (M. grisea); race-specific resistance; different pathogenic isolates
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Ma, S.; Lin, S.; Wang, M.; Zou, Y.; Tao, H.; Liu, W.; Zhang, L.; Liang, K.; Ai, Y.; He, H. Differential Expression Proteins Contribute to Race-Specific Resistant Ability in Rice (Oryza sativa L.). Plants 2019, 8, 29.
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