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Plants 2018, 7(4), 99; https://doi.org/10.3390/plants7040099

Developmental Stage and Shape of Embryo Determine the Efficacy of Embryo Rescue in Introgressing Orange/Yellow Color and Anthocyanin Genes of Brassica Species

1
Department of Horticulture, Suncheon National University, 255 Jungang-ro, Suncheon, Jeonnam 57922, Korea
2
Department of Genetics and Plant Breeding, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh
3
Center for Horticultural Seed Development of Golden Seed Project (GSP), Suncheon National University, 255 Jungang-ro, Suncheon, Jeonnam 57922, Korea
4
University-Industry Cooperation Foundation, Suncheon National University, 255 Jungang-ro, Suncheon, Jeonnam 57922, Korea
Authors contributed equally.
*
Author to whom correspondence should be addressed.
Received: 16 October 2018 / Revised: 7 November 2018 / Accepted: 9 November 2018 / Published: 13 November 2018
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Abstract

Vegetables in Brassica are some of the world’s most commonly cultivated plants and have a wide range of consumable plant organs. Improvement of this group of vegetables is limited at the species level due to limited genetic variability. Interspecies hybridization could be a powerful alternate tool for broadening the genetic variability of target traits. Embryo rescue technique is necessarily practiced in interspecies hybridization for protecting embryos from premature abortion. However, its success depends on the age of ovaries, shape of embryos, and the effect of female genotype. In this study, we carried out a wide range of interspecies crossing for introgressing target traits (orange/yellow color in cabbage and anthocyanin in Chinese cabbage) and optimizing the appropriate age of ovaries, the shape of embryo, and the suitable genotypes of such crosses. We observed that 15 DAP (days after pollination) was the best for embryo rescue in the diploid-diploid (Brassica rapa × B. oleracea) crosses, while 20 DAP was optimum for amphidiploid-diploid (B. napus/B. juncea × B. rapa) crosses. Cotyledonary shape of embryos and genotypes of amphidiploid species were the best for successful plant regeneration in interspecies crosses. We successfully selected plants with desired orange/yellow inner leaves for cabbage and higher anthocyanin in Chinese cabbage. The results of this study have the potential to be applied for the efficient production of interspecific hybrids and to develop Brassica vegetables with new traits, which could have potential for the enrichment of the human diet. View Full-Text
Keywords: interspecies hybridization; orange color; cabbage; Chinese cabbage interspecies hybridization; orange color; cabbage; Chinese cabbage
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Pen, S.; Nath, U.K.; Song, S.; Goswami, G.; Lee, J.-H.; Jung, H.-J.; Kim, H.-T.; Park, J.-I.; Nou, I.-S. Developmental Stage and Shape of Embryo Determine the Efficacy of Embryo Rescue in Introgressing Orange/Yellow Color and Anthocyanin Genes of Brassica Species. Plants 2018, 7, 99.

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