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Article

FvHsfB1a Gene Improves Thermotolerance in Transgenic Arabidopsis

1
School of Horticulture, Anhui Agricultural University, Hefei 230036, China
2
Shanghai Agricultural Technology Extension Service Center, Shanghai 201103, China
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Plants 2025, 14(15), 2392; https://doi.org/10.3390/plants14152392 (registering DOI)
Submission received: 27 June 2025 / Revised: 29 July 2025 / Accepted: 31 July 2025 / Published: 2 August 2025
(This article belongs to the Special Issue Cell Physiology and Stress Adaptation of Crops)

Abstract

 Heat stress transcription factor (Hsf) families play important roles in abiotic stress responses. However, previous studies reported that HsfBs genes may play diverse roles in response to heat stress. Here, we conducted functional analysis on a woodland strawberry Class B Hsf gene, FvHsfB1a, to improve thermotolerance. The structure of FvHsfB1a contains a typical Hsf domain for DNA binding at the N-terminus, and FvHsfB1a belongs to the B1 family of Hsfs. The FvHsfB1a protein was localized in the nucleus. The FvHsfB1a gene was expressed in various strawberry tissues and highly induced by heat treatment. Under heat stress conditions, ectopic expression of FvHsfB1a in Arabidopsis improves thermotolerance, with higher germination and survival rates, a longer primary root length, higher proline and chlorophyll contents, lower malonaldehyde (MDA) and O2− contents, better enzyme activities, and greater expression of heat-responsive and stress-related genes compared to WT. FvWRKY75 activates the promoter of the FvHsfB1a gene through recognizing the W-box element. Similarly, FvWRKY75-OE lines also displayed a heat-tolerant phenotype, exhibiting more proline and chlorophyll contents, lower MDA and O2− contents, and higher enzyme activities under heat stress. Taken together, our study indicates that FvHsfB1a is a positive regulator of heat stress. 
Keywords: strawberry; FvHsfB1a; FvWRKY75; heat stress; Arabidopsis thaliana strawberry; FvHsfB1a; FvWRKY75; heat stress; Arabidopsis thaliana

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MDPI and ACS Style

Cao, Q.; Mao, T.; Yang, K.; Xie, H.; Li, S.; Xue, H. FvHsfB1a Gene Improves Thermotolerance in Transgenic Arabidopsis. Plants 2025, 14, 2392. https://doi.org/10.3390/plants14152392

AMA Style

Cao Q, Mao T, Yang K, Xie H, Li S, Xue H. FvHsfB1a Gene Improves Thermotolerance in Transgenic Arabidopsis. Plants. 2025; 14(15):2392. https://doi.org/10.3390/plants14152392

Chicago/Turabian Style

Cao, Qian, Tingting Mao, Kebang Yang, Hanxiu Xie, Shan Li, and Hao Xue. 2025. "FvHsfB1a Gene Improves Thermotolerance in Transgenic Arabidopsis" Plants 14, no. 15: 2392. https://doi.org/10.3390/plants14152392

APA Style

Cao, Q., Mao, T., Yang, K., Xie, H., Li, S., & Xue, H. (2025). FvHsfB1a Gene Improves Thermotolerance in Transgenic Arabidopsis. Plants, 14(15), 2392. https://doi.org/10.3390/plants14152392

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