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Article

Growth under Different Trophic Regimes and Synchronization of the Red Microalga Galdieria sulphuraria

1
Centre Algatech, Laboratory of Cell Cycles of Algae, Institute of Microbiology of the Czech Academy of Sciences, Novohradská 237, 379 81 Třeboň, Czech Republic
2
Faculty of Science, University of South Bohemia, Branišovská 1760, 370 05 České Budějovice, Czech Republic
3
Department of Medical and Pharmaceutical Biotechnology, IMC University of Applied Sciences, Piaristengasse 1, 3500 Krems, Austria
4
Institute of Microbiology of the Czech Academy of Sciences, Vídeňská 1083, 142 20 Prague, Czech Republic
*
Author to whom correspondence should be addressed.
These two authors contributed to the study equally.
Academic Editor: Vladimir N. Uversky
Biomolecules 2021, 11(7), 939; https://doi.org/10.3390/biom11070939
Received: 14 May 2021 / Revised: 21 June 2021 / Accepted: 22 June 2021 / Published: 24 June 2021
(This article belongs to the Special Issue Cell Cycle Regulation of Algae)
The extremophilic unicellular red microalga Galdieria sulphuraria (Cyanidiophyceae) is able to grow autotrophically, or mixo- and heterotrophically with 1% glycerol as a carbon source. The alga divides by multiple fission into more than two cells within one cell cycle. The optimal conditions of light, temperature and pH (500 µmol photons m−2 s−1, 40 °C, and pH 3; respectively) for the strain Galdieria sulphuraria (Galdieri) Merola 002 were determined as a basis for synchronization experiments. For synchronization, the specific light/dark cycle, 16/8 h was identified as the precondition for investigating the cell cycle. The alga was successfully synchronized and the cell cycle was evaluated. G. sulphuraria attained two commitment points with midpoints at 10 and 13 h of the cell cycle, leading to two nuclear divisions, followed subsequently by division into four daughter cells. The daughter cells stayed in the mother cell wall until the beginning of the next light phase, when they were released. Accumulation of glycogen throughout the cell cycle was also described. The findings presented here bring a new contribution to our general understanding of the cell cycle in cyanidialean red algae, and specifically of the biotechnologically important species G. sulphuraria. View Full-Text
Keywords: cell cycle; red algae; Galdieria; growth; cell division; light intensity; temperature; trophic regimes; synchronization cell cycle; red algae; Galdieria; growth; cell division; light intensity; temperature; trophic regimes; synchronization
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MDPI and ACS Style

Náhlík, V.; Zachleder, V.; Čížková, M.; Bišová, K.; Singh, A.; Mezricky, D.; Řezanka, T.; Vítová, M. Growth under Different Trophic Regimes and Synchronization of the Red Microalga Galdieria sulphuraria. Biomolecules 2021, 11, 939. https://doi.org/10.3390/biom11070939

AMA Style

Náhlík V, Zachleder V, Čížková M, Bišová K, Singh A, Mezricky D, Řezanka T, Vítová M. Growth under Different Trophic Regimes and Synchronization of the Red Microalga Galdieria sulphuraria. Biomolecules. 2021; 11(7):939. https://doi.org/10.3390/biom11070939

Chicago/Turabian Style

Náhlík, Vít, Vilém Zachleder, Mária Čížková, Kateřina Bišová, Anjali Singh, Dana Mezricky, Tomáš Řezanka, and Milada Vítová. 2021. "Growth under Different Trophic Regimes and Synchronization of the Red Microalga Galdieria sulphuraria" Biomolecules 11, no. 7: 939. https://doi.org/10.3390/biom11070939

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