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Article

Elastomeric Electrospun Scaffolds of a Biodegradable Aliphatic Copolyester Containing PEG-Like Sequences for Dynamic Culture of Human Endothelial Cells

1
Tissuegraft s.r.l., 28100 Novara, Italy
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Department of Chemistry “Giacomo Ciamician” and INSTM UdR of Bologna, University of Bologna, 40126 Bologna, Italy
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Interdepartmental Center for Industrial Research on Advanced Applications in Mechanical Engineering and Materials Technology, CIRI-MAM, University of Bologna, 40136 Bologna, Italy
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Department of Science and Technological Innovation and INSTM UdR Alessandria, University of Piemonte Orientale, 15121 Alessandria, Italy
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Department of Civil, Chemical, Environmental and Materials Engineering, University of Bologna, 40131 Bologna, Italy
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Department of Health Sciences, University of Piemonte Orientale, 28100 Novara, Italy
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Health Sciences & Technologies (HST) CIRI, University of Bologna, 40064 Ozzano dell’Emilia, Italy
*
Authors to whom correspondence should be addressed.
Biomolecules 2020, 10(12), 1620; https://doi.org/10.3390/biom10121620
Received: 9 November 2020 / Revised: 26 November 2020 / Accepted: 27 November 2020 / Published: 30 November 2020
(This article belongs to the Special Issue Biodegradable Polyesters: From Synthesis to Application)
In the field of artificial prostheses for damaged vessel replacement, polymeric scaffolds showing the right combination of mechanical performance, biocompatibility, and biodegradability are still demanded. In the present work, poly(butylene-co-triethylene trans-1,4-cyclohexanedicarboxylate), a biodegradable random aliphatic copolyester, has been synthesized and electrospun in form of aligned and random fibers properly designed for vascular applications. The obtained materials were analyzed through tensile and dynamic-mechanical tests, the latter performed under conditions simulating the mechanical contraction of vascular tissue. Furthermore, the in vitro biological characterization, in terms of hemocompatibility and cytocompatibility in static and dynamic conditions, was also carried out. The mechanical properties of the investigated scaffolds fit within the range of physiological properties for medium- and small-caliber blood vessels, and the aligned scaffolds displayed a strain-stiffening behavior typical of the blood vessels. Furthermore, all the produced scaffolds showed constant storage and loss moduli in the investigated timeframe (24 h), demonstrating the stability of the scaffolds under the applied conditions of mechanical deformation. The biological characterization highlighted that the mats showed high hemocompatibility and low probability of thrombus formation; finally, the cytocompatibility tests demonstrated that cyclic stretch of electrospun fibers increased endothelial cell activity and proliferation, in particular on aligned scaffolds. View Full-Text
Keywords: poly(butylene-co-triethylene trans-1,4-cyclohexanedicarboxylate); electrospinning; mechanical characterization; hemocompatibility assay; endothelial cells; elastomeric scaffold; artificial prosthesis; vascular tissue engineering; dynamic cell culture poly(butylene-co-triethylene trans-1,4-cyclohexanedicarboxylate); electrospinning; mechanical characterization; hemocompatibility assay; endothelial cells; elastomeric scaffold; artificial prosthesis; vascular tissue engineering; dynamic cell culture
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MDPI and ACS Style

Fusaro, L.; Gualandi, C.; Antonioli, D.; Soccio, M.; Liguori, A.; Laus, M.; Lotti, N.; Boccafoschi, F.; Focarete, M.L. Elastomeric Electrospun Scaffolds of a Biodegradable Aliphatic Copolyester Containing PEG-Like Sequences for Dynamic Culture of Human Endothelial Cells. Biomolecules 2020, 10, 1620. https://doi.org/10.3390/biom10121620

AMA Style

Fusaro L, Gualandi C, Antonioli D, Soccio M, Liguori A, Laus M, Lotti N, Boccafoschi F, Focarete ML. Elastomeric Electrospun Scaffolds of a Biodegradable Aliphatic Copolyester Containing PEG-Like Sequences for Dynamic Culture of Human Endothelial Cells. Biomolecules. 2020; 10(12):1620. https://doi.org/10.3390/biom10121620

Chicago/Turabian Style

Fusaro, Luca, Chiara Gualandi, Diego Antonioli, Michelina Soccio, Anna Liguori, Michele Laus, Nadia Lotti, Francesca Boccafoschi, and Maria Letizia Focarete. 2020. "Elastomeric Electrospun Scaffolds of a Biodegradable Aliphatic Copolyester Containing PEG-Like Sequences for Dynamic Culture of Human Endothelial Cells" Biomolecules 10, no. 12: 1620. https://doi.org/10.3390/biom10121620

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