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Open AccessArticle

Ammonium Fluoride as Suitable Additive for HILIC-Based LC-HRMS Metabolomics

Laboratoire d’Etude des Résidus et Contaminants dans les Aliments (LABERCA), Oniris, INRA, F-44307 Nantes, France
Département des analyses, Agence Française de Lutte contre le Dopage (AFLD), 92290 Châtenay-Malabry, France
Author to whom correspondence should be addressed.
Metabolites 2019, 9(12), 292;
Received: 28 October 2019 / Revised: 19 November 2019 / Accepted: 23 November 2019 / Published: 27 November 2019
(This article belongs to the Special Issue Metabolomics 2019)
Hydrophilic Interaction Liquid Chromatography (HILIC) chromatography is widely applied in metabolomics as a complementary strategy to reverse phase chromatography. Nevertheless, it still faces several issues in terms of peak shape and compounds ionization, limiting the automatic de-convolution and data semi-quantification performed through dedicated software. A way to improve the chromatographic and ionization performance of a HILIC method is to modify the electrostatic interactions of the analytes with both mobile and stationary phases. In this study, using a ZIC-HILIC chromatographic phase, we evaluated the performance of ammonium fluoride (AF) as additive salt, comparing its performance to ammonium acetate (AA). Three comparative criteria were selected: (1) identification and peak quality of 34 standards following a metabolomics-specific evaluation approach, (2) an intraday repeatability test with real samples and (3) performing two real metabolomics fingerprints with the AF method to evaluate its inter-day repeatability. The AF method showed not only higher ionization efficiency and signal-to-noise ratio but also better repeatability and robustness than the AA approach. A tips and tricks section is then added, aiming at improving method replicability for further users. In conclusion, ammonium fluoride as additive salt presents several advantages and might be considered as a step forward in the application of robust HILIC methods in metabolomics. View Full-Text
Keywords: HILIC; metabolomics; mobile phase modifier; ammonium fluoride HILIC; metabolomics; mobile phase modifier; ammonium fluoride
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Narduzzi, L.; Royer, A.-L.; Bichon, E.; Guitton, Y.; Buisson, C.; Le Bizec, B.; Dervilly-Pinel, G. Ammonium Fluoride as Suitable Additive for HILIC-Based LC-HRMS Metabolomics. Metabolites 2019, 9, 292.

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