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Open AccessArticle

Mucosal Metabolomic Profiling and Pathway Analysis Reveal the Metabolic Signature of Ulcerative Colitis

1
Natural Products and Medicinal Chemistry Research Group, Department of Pharmacy, Faculty of Health Sciences, University of Tromsø The Arctic University of Norway, 9037 Tromsø, Norway
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Research Group of Gastroenterology and Nutrition, Department of Clinical Medicine, Faculty of Health Sciences, University of Tromsø The Arctic University of Norway, 9037 Tromsø, Norway
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Department of Medical Gastroenterology, University Hospital of North Norway, 9037 Tromsø, Norway
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Swedish Metabolomics Center, Department of Molecular Biology, Umeå University, 90736 Umeå, Sweden
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The Novo Nordisk Foundation Center for Basic Metabolic Research, 2200 Copenhagen, Denmark
*
Author to whom correspondence should be addressed.
Metabolites 2019, 9(12), 291; https://doi.org/10.3390/metabo9120291
Received: 15 October 2019 / Revised: 21 November 2019 / Accepted: 25 November 2019 / Published: 27 November 2019
The onset of ulcerative colitis (UC) is characterized by a dysregulated mucosal immune response triggered by several genetic and environmental factors in the context of host–microbe interaction. This complexity makes UC ideal for metabolomic studies to unravel the disease pathobiology and to improve the patient stratification strategies. This study aims to explore the mucosal metabolomic profile in UC patients, and to define the UC metabolic signature. Treatment- naïve UC patients (n = 18), UC patients in deep remission (n = 10), and healthy volunteers (n = 14) were recruited. Mucosa biopsies were collected during colonoscopies. Metabolomic analysis was performed by combined gas chromatography coupled to time-of-flight mass spectrometry (GC-TOF-MS) and ultra-high performance liquid chromatography coupled with mass spectrometry (UHPLC-MS). In total, 177 metabolites from 50 metabolic pathways were identified. The most prominent metabolome changes among the study groups were in lysophosphatidylcholine, acyl carnitine, and amino acid profiles. Several pathways were found perturbed according to the integrated pathway analysis. These pathways ranged from amino acid metabolism (such as tryptophan metabolism) to fatty acid metabolism, namely linoleic and butyrate. These metabolic changes during UC reflect the homeostatic disturbance in the gut, and highlight the importance of system biology approaches to identify key drivers of pathogenesis which prerequisite personalized medicine. View Full-Text
Keywords: inflammatory bowel disease; metabolomics; pathway analysis; ulcerative colitis; tryptophan metabolism; fatty acid metabolism; personalized treatment inflammatory bowel disease; metabolomics; pathway analysis; ulcerative colitis; tryptophan metabolism; fatty acid metabolism; personalized treatment
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Diab, J.; Hansen, T.; Goll, R.; Stenlund, H.; Jensen, E.; Moritz, T.; Florholmen, J.; Forsdahl, G. Mucosal Metabolomic Profiling and Pathway Analysis Reveal the Metabolic Signature of Ulcerative Colitis. Metabolites 2019, 9, 291.

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