Next Article in Journal
The Variability of Thymol and Carvacrol Contents Reveals the Level of Antibacterial Activity of the Essential Oils from Different Accessions of Oliveria decumbens
Next Article in Special Issue
Bacteriophages and the One Health Approach to Combat Multidrug Resistance: Is This the Way?
Previous Article in Journal
Substandard Quality of the Antimicrobials Sold in the Street Markets in Haiti
Previous Article in Special Issue
Tackling Intrinsic Antibiotic Resistance in Serratia marcescens with a Combination of Ampicillin/Sulbactam and Phage SALSA
Article

Kinetic Fingerprinting Links Bacteria-Phage Interactions with Emergent Dynamics: Rapid Depletion of Klebsiella pneumoniae Indicates Phage Synergy

1
Paul-Ehrlich-Institut, 63225 Langen, Germany
2
Goethe University Frankfurt, Institute of Mathematics, 60325 Frankfurt, Germany
3
Leibniz Institute DSMZ German Collection of Microorganisms and Cell Cultures, 38124 Braunschweig, Germany
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Antibiotics 2020, 9(7), 408; https://doi.org/10.3390/antibiotics9070408
Received: 4 June 2020 / Revised: 1 July 2020 / Accepted: 6 July 2020 / Published: 14 July 2020
(This article belongs to the Special Issue Phage Diversity for Research and Application)
The specific temporal evolution of bacterial and phage population sizes, in particular bacterial depletion and the emergence of a resistant bacterial population, can be seen as a kinetic fingerprint that depends on the manifold interactions of the specific phage–host pair during the course of infection. We have elaborated such a kinetic fingerprint for a human urinary tract Klebsiella pneumoniae isolate and its phage vB_KpnP_Lessing by a modeling approach based on data from in vitro co-culture. We found a faster depletion of the initially sensitive bacterial population than expected from simple mass action kinetics. A possible explanation for the rapid decline of the bacterial population is a synergistic interaction of phages which can be a favorable feature for phage therapies. In addition to this interaction characteristic, analysis of the kinetic fingerprint of this bacteria and phage combination revealed several relevant aspects of their population dynamics: A reduction of the bacterial concentration can be achieved only at high multiplicity of infection whereas bacterial extinction is hardly accomplished. Furthermore the binding affinity of the phage to bacteria is identified as one of the most crucial parameters for the reduction of the bacterial population size. Thus, kinetic fingerprinting can be used to infer phage–host interactions and to explore emergent dynamics which facilitates a rational design of phage therapies. View Full-Text
Keywords: population dynamics; mathematical modeling; Klebsiella pneumoniae; phage; emergence; resistance; synergistic interaction; kinetic fingerprint; phage therapy population dynamics; mathematical modeling; Klebsiella pneumoniae; phage; emergence; resistance; synergistic interaction; kinetic fingerprint; phage therapy
Show Figures

Figure 1

MDPI and ACS Style

Loessner, H.; Schlattmeier, I.; Anders-Maurer, M.; Bekeredjian-Ding, I.; Rohde, C.; Wittmann, J.; Pokalyuk, C.; Krut, O.; Kamp, C. Kinetic Fingerprinting Links Bacteria-Phage Interactions with Emergent Dynamics: Rapid Depletion of Klebsiella pneumoniae Indicates Phage Synergy. Antibiotics 2020, 9, 408. https://doi.org/10.3390/antibiotics9070408

AMA Style

Loessner H, Schlattmeier I, Anders-Maurer M, Bekeredjian-Ding I, Rohde C, Wittmann J, Pokalyuk C, Krut O, Kamp C. Kinetic Fingerprinting Links Bacteria-Phage Interactions with Emergent Dynamics: Rapid Depletion of Klebsiella pneumoniae Indicates Phage Synergy. Antibiotics. 2020; 9(7):408. https://doi.org/10.3390/antibiotics9070408

Chicago/Turabian Style

Loessner, Holger, Insea Schlattmeier, Marie Anders-Maurer, Isabelle Bekeredjian-Ding, Christine Rohde, Johannes Wittmann, Cornelia Pokalyuk, Oleg Krut, and Christel Kamp. 2020. "Kinetic Fingerprinting Links Bacteria-Phage Interactions with Emergent Dynamics: Rapid Depletion of Klebsiella pneumoniae Indicates Phage Synergy" Antibiotics 9, no. 7: 408. https://doi.org/10.3390/antibiotics9070408

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop