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Biosensors 2016, 6(2), 22;

A Flow SPR Immunosensor Based on a Sandwich Direct Method

Department of Chemistry, Sapienza University of Rome, P.le Aldo Moro 5, 00185 Rome, Italy
Department of Chemistry and Drug Technologies, Sapienza University of Rome, P.le Aldo Moro 5, 00185 Rome, Italy
Author to whom correspondence should be addressed.
Academic Editor: Nicole Jaffrezic-Renault
Received: 2 March 2016 / Revised: 13 April 2016 / Accepted: 4 May 2016 / Published: 13 May 2016
(This article belongs to the Special Issue Next-Generation Immunosensors)
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In this study, we report the development of an SPR (Surface Plasmon Resonance) immunosensor for the detection of ampicillin, operating under flow conditions. SPR sensors based on both direct (with the immobilization of the antibody) and competitive (with the immobilization of the antigen) methods did not allow the detection of ampicillin. Therefore, a sandwich-based sensor was developed which showed a good linear response towards ampicillin between 10−3 and 10−1 M, a measurement time of ≤20 min and a high selectivity both towards β-lactam antibiotics and antibiotics of different classes. View Full-Text
Keywords: ampicillin; flow immunosensor SPR; sandwich method ampicillin; flow immunosensor SPR; sandwich method

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Tomassetti, M.; Conta, G.; Campanella, L.; Favero, G.; Sanzò, G.; Mazzei, F.; Antiochia, R. A Flow SPR Immunosensor Based on a Sandwich Direct Method. Biosensors 2016, 6, 22.

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