Medium-Dependent Antibacterial Properties and Bacterial Filtration Ability of Reduced Graphene Oxide
by
Alexander Gusev 1,2, Olga Zakharova 1,2, Dmitry S. Muratov 2, Nataliia S. Vorobeva 3, Mamun Sarker 3, Iaroslav Rybkin 4, Daniil Bratashov 4
, Evgeny Kolesnikov 2, Aleš Lapanje 4,5, Denis V. Kuznetsov 2 and Alexander Sinitskii 3,6,*
Alexander Gusev 1,2, Olga Zakharova 1,2, Dmitry S. Muratov 2, Nataliia S. Vorobeva 3, Mamun Sarker 3, Iaroslav Rybkin 4, Daniil Bratashov 4, Evgeny Kolesnikov 2, Aleš Lapanje 4,5, Denis V. Kuznetsov 2 and Alexander Sinitskii 3,6,*
1
Research Institute for Environmental Science and Biotechnology, Derzhavin Tambov State University, Tambov 392000, Russia
2
National University of Science and Technology “MISIS”, Moscow 119991, Russia
3
Department of Chemistry, University of Nebraska—Lincoln, Lincoln, NE 68588, USA
4
Remote Controlled Theranostic Systems Lab, Saratov State University, Saratov 410012, Russia
5
Jožef Stefan Institute, 1000 Ljubljana, Slovenia
6
Nebraska Center for Materials and Nanoscience, University of Nebraska—Lincoln, Lincoln, NE 68588, USA
*
Author to whom correspondence should be addressed.
Nanomaterials 2019, 9(10), 1454; https://doi.org/10.3390/nano9101454
Received: 28 August 2019 / Revised: 8 October 2019 / Accepted: 11 October 2019 / Published: 13 October 2019
(This article belongs to the Special Issue Nanopharmaceutics)
Toxicity of reduced graphene oxide (rGO) has been a topic of multiple studies and was shown to depend on a variety of characteristics of rGO and biological objects of interest. In this paper, we demonstrate that when studying the same dispersions of rGO and fluorescent Escherichia coli (E. coli) bacteria, the outcome of nanotoxicity experiments also depends on the type of culture medium. We show that rGO inhibits the growth of bacteria in a nutrition medium but shows little effect on the behavior of E. coli in a physiological saline solution. The observed effects of rGO on E. coli in different media could be at least partially rationalized through the adsorption of bacteria and nutrients on the dispersed rGO sheets, which is likely mediated via hydrogen bonding. We also found that the interaction between rGO and E. coli is medium-dependent, and in physiological saline solutions they form stable flocculate structures that were not observed in nutrition media. Furthermore, the aggregation of rGO and E. coli in saline media was observed regardless of whether the bacteria were alive or dead. Filtration of the aggregate suspensions led to nearly complete removal of bacteria from filtered liquids, which highlights the potential of rGO for the filtration and separation of biological contaminants, regardless of whether they include live or dead microorganisms.
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MDPI and ACS Style
Gusev, A.; Zakharova, O.; Muratov, D.S.; Vorobeva, N.S.; Sarker, M.; Rybkin, I.; Bratashov, D.; Kolesnikov, E.; Lapanje, A.; Kuznetsov, D.V.; Sinitskii, A. Medium-Dependent Antibacterial Properties and Bacterial Filtration Ability of Reduced Graphene Oxide. Nanomaterials 2019, 9, 1454.
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