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Article

Fish Oil Nanoemulsion Supplementation Attenuates Bleomycin-Induced Pulmonary Fibrosis BALB/c Mice

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Nanobiotechnology Laboratory, Genetics & Morphology Department, Institute of Biological Science, University of Brasília, Brasília 70910-900, Brazil
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Applied Immunology Laboratory, Cell Biology Department, Institute of Biological Science, University of Brasília, Brasília 70910-900, Brazil
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Respiratory Physiology Laboratory, Faculty of Medicine, University of Brasília, Brasília 70910-900, Brazil
*
Author to whom correspondence should be addressed.
Academic Editor: Rosalia Bertorelli
Nanomaterials 2022, 12(10), 1683; https://doi.org/10.3390/nano12101683
Received: 16 March 2022 / Revised: 27 April 2022 / Accepted: 5 May 2022 / Published: 14 May 2022
(This article belongs to the Special Issue Nanomaterials in Biological Systems: Opportunities and Challenges)
Diets rich in omega-3 or -6 fatty acids will produce different profiles for cell membranes phospholipid constitutions. Omegas 3 and 6 are part of the diet and can modulate the inflammatory profile. We evaluated the effects of the oral absorption of fish oil, when associated with a lipid nanoemulsion in an experimental pulmonary inflammatory model. Pulmonary fibrosis is a disease associated with excessive extracellular matrix deposition. We determined to investigate the morphophysiological mechanisms in mice that were pretreated after induction with bleomycin (BLM). The pretreatment was for 21 days with saline solution, sunflower oil (SO), fish oil (FO), and fish oil nanoemulsion (NEW3). The animals received a daily dose of 50 mg/Kg of docosahexaenoic acid DHA and 10 mg/Kg eicosapentaenoic (EPA) (100 mg/Kg), represented by a daily dose of 40 µL of NEW3. The blank group was treated with the same amount daily (40 µL) during the 21 days of pretreatment. The animals were treated with SO and FO, 100 mg/Kg (containing 58 mg/Kg of polyunsaturated fats/higher% linoleic acid) and 100 mg/Kg (50 mg/Kg of DHA and 10 mg/Kg EPA), respectively. A single dose of 5 mg/mL (50 μL) bleomycin sulfate, by the intratracheal surgical method in BALB/cAnNTac (BALB/c). NEW3 significantly reduced fibrotic progression, which can be evidenced by the protection from loss of body mass, increase in respiratory incursions per minute, decreased spacing of alveolar septa, decreased severity of fibrosis, and changes in the respiratory system. NEW3 attenuated the inflammatory changes developed in the experimental model of pulmonary fibrosis, while group SO showed a significant increase in inflammatory changes. This concluded that the presented results demonstrated that is possible to positively modulate the immune and inflamamtory response to an external agressor, by changing the nutitional intake of specific fatty acids, such as omega-3 placed in fish oil. Moreover, these benefits can be improved by the nanoencapsulation of fish oil in lipid nanoemulsions. View Full-Text
Keywords: nanoemulsion; lung fibrosis; bleomycin; fish oil nanoemulsion; lung fibrosis; bleomycin; fish oil
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MDPI and ACS Style

Galdino de Souza, D.; Santos, D.S.; Simon, K.S.; Morais, J.A.V.; Coelho, L.C.; Pacheco, T.J.A.; Azevedo, R.B.; Bocca, A.L.; Melo-Silva, C.A.; Longo, J.P.F. Fish Oil Nanoemulsion Supplementation Attenuates Bleomycin-Induced Pulmonary Fibrosis BALB/c Mice. Nanomaterials 2022, 12, 1683. https://doi.org/10.3390/nano12101683

AMA Style

Galdino de Souza D, Santos DS, Simon KS, Morais JAV, Coelho LC, Pacheco TJA, Azevedo RB, Bocca AL, Melo-Silva CA, Longo JPF. Fish Oil Nanoemulsion Supplementation Attenuates Bleomycin-Induced Pulmonary Fibrosis BALB/c Mice. Nanomaterials. 2022; 12(10):1683. https://doi.org/10.3390/nano12101683

Chicago/Turabian Style

Galdino de Souza, Danielle, Débora S. Santos, Karina S. Simon, José A.V. Morais, Luísa C. Coelho, Thyago J.A. Pacheco, Ricardo B. Azevedo, Anamélia L. Bocca, César A. Melo-Silva, and João P.F. Longo. 2022. "Fish Oil Nanoemulsion Supplementation Attenuates Bleomycin-Induced Pulmonary Fibrosis BALB/c Mice" Nanomaterials 12, no. 10: 1683. https://doi.org/10.3390/nano12101683

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