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Open AccessReview

Genotoxicity of Silver Nanoparticles

Department of Pharmacology and Toxicology, Faculty of Pharmacy and Nutrition, Universidad de Navarra, Irunlarrea 1, 31008 Pamplona, Spain
Navarra Institute for Health Research (IdiSNA), 31008 Pamplona, Spain
Author to whom correspondence should be addressed.
Nanomaterials 2020, 10(2), 251;
Received: 27 November 2019 / Revised: 22 January 2020 / Accepted: 24 January 2020 / Published: 31 January 2020
Silver nanoparticles (AgNPs) are widely used in diverse sectors such as medicine, food, cosmetics, household items, textiles and electronics. Given the extent of human exposure to AgNPs, information about the toxicological effects of such products is required to ensure their safety. For this reason, we performed a bibliographic review of the genotoxicity studies carried out with AgNPs over the last six years. A total of 43 articles that used well-established standard assays (i.e., in vitro mouse lymphoma assays, in vitro micronucleus tests, in vitro comet assays, in vivo micronucleus tests, in vivo chromosome aberration tests and in vivo comet assays), were selected. The results showed that AgNPs produce genotoxic effects at all DNA damage levels evaluated, in both in vitro and in vivo assays. However, a higher proportion of positive results was obtained in the in vitro studies. Some authors observed that coating and size had an effect on both in vitro and in vivo results. None of the studies included a complete battery of assays, as recommended by ICH and EFSA guidelines, and few of the authors followed OECD guidelines when performing assays. A complete genotoxicological characterization of AgNPs is required for decision-making.
Keywords: silver nanoparticles; genotoxicity; in vitro; in vivo; mouse lymphoma assay; micronucleus test; chromosome aberration test; comet assay silver nanoparticles; genotoxicity; in vitro; in vivo; mouse lymphoma assay; micronucleus test; chromosome aberration test; comet assay
MDPI and ACS Style

Rodriguez-Garraus, A.; Azqueta, A.; Vettorazzi, A.; López de Cerain, A. Genotoxicity of Silver Nanoparticles. Nanomaterials 2020, 10, 251.

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