Reference Intervals of Thyroid Function Tests Assessed by Immunoassay and Mass Spectrometry in Healthy Pregnant Women Living in Catalonia
Abstract
:1. Introduction
2. Material and Methods
2.1. Study Design and Patients
2.2. Laboratory Procedures
2.3. Liquid Chromatography-Mass Spectrometry (LC/MS/MS)
2.3.1. Chemicals, Reagents, and Standards
2.3.2. Solutions and Standards
2.3.3. Sample Preparation
2.3.4. LC/MS/MS Setup
2.4. Statistics Analysis
3. Results
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Acknowledgments
Conflicts of Interest
References
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First Trimester | Second Trimester | Third Trimester | p# | |
---|---|---|---|---|
•Number | 270 | 212 | 211 | |
•Maternal age (years) *& | 32.3 ± 5.2 | |||
•Maternal weight (kg) *& | 64.5 ± 13.6 | |||
•Maternal BMI (kg/m2) *& | 24.8 ± 4.9 | |||
•Parity (%) | ||||
-First gestation | 48 | |||
-Second gestation | 41 | |||
-Third or more gestation | 11 | |||
•Previous miscarriages (%) | ||||
-None | 61 | |||
-One | 29 | |||
-Two or more | 10 | |||
•Gestational age (weeks) && | 9–11 | 24–28 | 29–33 | |
•Level of education (%) | ||||
-None/primary | 25.3 | |||
-Secondary | 49.6 | |||
-Higher education | 25.1 | |||
•Smoking habit (%) & | ||||
-Nonsmoker | 80.2 | |||
-Smoker | 18.2 | |||
•Working women (%) | 81.2 | |||
•Consumption of iodized salt (%) & | 25.3% | |||
•Use of supplements (%) & | ||||
-None | 46 | |||
-Potassium iodide | 28.6 | |||
-Multivitamins | 25.4 | |||
TPO-Ab ** (UI/mL) | 0.55 (0.5–1.37) | 0.57 (0.5–3.09) | 0.55 (0.5–2.66) | NS |
Tg-Ab (UI/mL) ** | 0.99 (0.4–9.7) | 1.04 (0.43–7.0) | 0.99 (0.38–5.4) | N.S: |
HCG (mUI/mL) ** | 110,583 (20,164–345,434 | |||
Albumin (g/L) ** | 40.2 (30.5–45) | 33.9 (30.3–38.4) a | 32.9 (29.1–37) a,b | <0.001 |
Creatinine ** (mg/dL) | 0.56 (0.43–0.73) | 0.52 (0.38–0.70) a | 0.5 (0.4–0.7a,b | <0.001 |
ALT (U/L) ** | 12 (6.58–40.85) | 12.0 (6.0–37.8) | 11 (6.0–50) | 0.129 |
Ferritin (ng/mL) ** | 42 (9–170) | 11.0 (4.8–80.5) a | 13 (4–46.8)a, b | <0.001 |
Thyroglobulin (ng/mL) ** | 15.3 (1.9–72.2) | 12.3 (1.9–81.1) a | 13.5 (2.4–86.6) a,b | <0.001 |
Urinary Iodide (µg/L) ** | 126.2 (32–402.3) | 178 (51.6–547) a | 170 (37.5–543) a,b | <0.001 |
Hb (g/dL) ** | 12.8 (10.9–14.5) | 11.4 (9.3–13.2) a | 11.6 (10.1–13.6) a,b | <0.001 |
Ht (%) ** | 37.9 (32.4–42.8) | 33.4 (27.9–38.6) a | 34.5 (29.9–40.5) a,b | <0.001 |
First Trimester | Second Trimester | Third Trimester | |
---|---|---|---|
(n = 270) | (n = 212) | (n = 211) | |
TSH Architect® | |||
Lower limit (90% CI) | 0.03 (0.02 to 0.11) a | 0.51 (0.34 to 0.62) b | 0.50 (0.38 to 0.67) |
Upper limit (90% CI) | 3.78 (3.26 to 4.71) | 3.53 (3.26 to 4.06) | 4.32 (3.52 to 4.72) |
FT4 Architect® | |||
Lower limit (90% CI) | 10.42 (10.04 to 10.55) a | 8.37 (8.37 to 8.88) b 12.74 (12.3.6 to 13.13) | 8.24 (7.85 to 8.62) |
Upper limit (90% CI) | 15.96 (15.32 to 16.6) | 12.49 (12.23 to 13.0) | |
FT4 Cobas® | |||
Lower limit (90% CI) | 11.46 (10.94 to 11.58) a,* | 9.65 (8.88 to 9.91) c | 8.88 (8.11 to 9.27) |
Upper limit (90% CI) | 19.05 (18.28 to 20.47) | 14.67 (14.16 to 15.83) | 14.54 (14.16 to 15.32) |
FT4 ID-LC/MS/MS | - | - | |
Lower limit (90% CI) | 8.75 (8.75 to 9.27) | ||
Upper limit (90% CI) | 18.27(17.12 to 19.44) |
First Trimester | Second Trimester | Third Trimester | |||||||
---|---|---|---|---|---|---|---|---|---|
Authors | n | w | FT4 (pmol/L) | n | w | FT4 (pmol/L) | n | w | FT4 (pmol/L) |
Hernandez JM et al. 2020 | 270 | <10 | 8.75–18.27 * | ||||||
Anckaert E et al. 2010 (10) | 29 | 12.6 ± 0.6 | 16.8 ± 2.2 # 12.49–21.1 ** | 33 | 25.3 ± 2.2 | 13.0 ± 1.8 # 12–14.2 ** | 34 | 36.1 ± 1.1 | 13.0 ± 2.3 # 11.0–14.8 ** |
Kahric-Janinc et al. 2007 (11) | 59 | 8.7 | 14.6 ± 2.96# 8.8–20.4 ** | 35 | 17.8 | 11.9 ± 3.9 # 4.3–19.5 ** | 26 | 28.7 | 11.1 ± 2.71 # 5.8–16.4 ** |
Yue et al. 2008 (24) | 72 120 | 14 20 | 13.9–15.2 * 11.1–19.7 * |
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Hernández, J.M.; Soldevila, B.; Velasco, I.; Moreno-Flores, F.; Ferrer, L.; Pérez-Montes de Oca, A.; Santillán, C.; Muñoz, C.; Ballesta, S.; Canal, C.; et al. Reference Intervals of Thyroid Function Tests Assessed by Immunoassay and Mass Spectrometry in Healthy Pregnant Women Living in Catalonia. J. Clin. Med. 2021, 10, 2444. https://doi.org/10.3390/jcm10112444
Hernández JM, Soldevila B, Velasco I, Moreno-Flores F, Ferrer L, Pérez-Montes de Oca A, Santillán C, Muñoz C, Ballesta S, Canal C, et al. Reference Intervals of Thyroid Function Tests Assessed by Immunoassay and Mass Spectrometry in Healthy Pregnant Women Living in Catalonia. Journal of Clinical Medicine. 2021; 10(11):2444. https://doi.org/10.3390/jcm10112444
Chicago/Turabian StyleHernández, José María, Berta Soldevila, Inés Velasco, Fernando Moreno-Flores, Laura Ferrer, Alejandra Pérez-Montes de Oca, Cecilia Santillán, Carla Muñoz, Sílvia Ballesta, Cristina Canal, and et al. 2021. "Reference Intervals of Thyroid Function Tests Assessed by Immunoassay and Mass Spectrometry in Healthy Pregnant Women Living in Catalonia" Journal of Clinical Medicine 10, no. 11: 2444. https://doi.org/10.3390/jcm10112444
APA StyleHernández, J. M., Soldevila, B., Velasco, I., Moreno-Flores, F., Ferrer, L., Pérez-Montes de Oca, A., Santillán, C., Muñoz, C., Ballesta, S., Canal, C., Puig-Domingo, M., & Granada, M. L. (2021). Reference Intervals of Thyroid Function Tests Assessed by Immunoassay and Mass Spectrometry in Healthy Pregnant Women Living in Catalonia. Journal of Clinical Medicine, 10(11), 2444. https://doi.org/10.3390/jcm10112444