Next Article in Journal
GspD, The Type II Secretion System Secretin of Leptospira, Protects Hamsters against Lethal Infection with a Virulent L. interrogans Isolate
Previous Article in Journal
Repertoires of Vaccine Refusal in Romania
Open AccessArticle

Lactobacilli Expressing Broadly Neutralizing Nanobodies against HIV-1 as Potential Vectors for HIV-1 Prophylaxis?

1
Georg-Speyer-Haus, Paul-Ehrlich-Straße 42-44, 60596 Frankfurt, Germany
2
Laboratory of Experimental Immunology, Institute of Virology, Faculty of Medicine and University Hospital Cologne, University of Cologne, 50931 Cologne, Germany
3
Department of Infectious Diseases, Virology Centre for Integrative Infectious Diseases Research (CIID), University Hospital Heidelberg, 69120 Heidelberg, Germany
4
Department of Laboratory Medicine, Division of Clinical Immunology and Transfusion Medicine, Karolinska Institutet at Karolinska University Hospital Huddinge, 14186 Stockholm, Sweden
*
Authors to whom correspondence should be addressed.
Current address: PRA Health Sciences, 68165 Mannheim, Germany.
Vaccines 2020, 8(4), 758; https://doi.org/10.3390/vaccines8040758
Received: 16 November 2020 / Revised: 7 December 2020 / Accepted: 11 December 2020 / Published: 13 December 2020
(This article belongs to the Section HIV Vaccines)
In the absence of an active prophylactic vaccine against HIV-1, passively administered, broadly neutralizing antibodies (bnAbs) identified in some chronically infected persons were shown to prevent HIV-1 infection in animal models. However, passive administration of bnAbs may not be suited to prevent sexual HIV-1 transmission in high-risk cohorts, as a continuous high level of active bnAbs may be difficult to achieve at the primary site of sexual transmission, the human vagina with its acidic pH. Therefore, we used Lactobacillus, a natural commensal in the healthy vaginal microbiome, to express bn nanobodies (VHH) against HIV-1 that we reported previously. After demonstrating that recombinant VHHA6 expressed in E. coli was able to protect humanized mice from mucosal infection by HIV-1Bal, we expressed VHHA6 in a soluble or in a cell-wall-anchored form in Lactobacillus rhamnosus DSM14870. This strain is already clinically applied for treatment of bacterial vaginosis. Both forms of VHHA6 neutralized a set of primary epidemiologically relevant HIV-1 strains in vitro. Furthermore, VHHA6 was still active at an acidic pH. Thus, lactobacilli expressing bn VHH potentially represent an attractive vector for the passive immunization of women in cohorts at high risk of HIV-1 transmission. View Full-Text
Keywords: nanobodies; VHH; prophylactic vaccine; neutralization; HIV-1; Lactobacillus; vector; humanized mouse model; passive immunization nanobodies; VHH; prophylactic vaccine; neutralization; HIV-1; Lactobacillus; vector; humanized mouse model; passive immunization
Show Figures

Figure 1

MDPI and ACS Style

Kalusche, S.; Vanshylla, K.; Kleipass, F.; Gruell, H.; Müller, B.; Zeng, Z.; Koch, K.; Stein, S.; Marcotte, H.; Klein, F.; Dietrich, U. Lactobacilli Expressing Broadly Neutralizing Nanobodies against HIV-1 as Potential Vectors for HIV-1 Prophylaxis? Vaccines 2020, 8, 758. https://doi.org/10.3390/vaccines8040758

AMA Style

Kalusche S, Vanshylla K, Kleipass F, Gruell H, Müller B, Zeng Z, Koch K, Stein S, Marcotte H, Klein F, Dietrich U. Lactobacilli Expressing Broadly Neutralizing Nanobodies against HIV-1 as Potential Vectors for HIV-1 Prophylaxis? Vaccines. 2020; 8(4):758. https://doi.org/10.3390/vaccines8040758

Chicago/Turabian Style

Kalusche, Sarah; Vanshylla, Kanika; Kleipass, Franziska; Gruell, Henning; Müller, Barbara; Zeng, Zhu; Koch, Kathrin; Stein, Stefan; Marcotte, Harold; Klein, Florian; Dietrich, Ursula. 2020. "Lactobacilli Expressing Broadly Neutralizing Nanobodies against HIV-1 as Potential Vectors for HIV-1 Prophylaxis?" Vaccines 8, no. 4: 758. https://doi.org/10.3390/vaccines8040758

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop