A Novel Pectic Polysaccharide of Jujube Pomace: Structural Analysis and Intracellular Antioxidant Activities
1
College of Food Science and Engineering, Northwest A&F University, Yangling 712100, China
2
Beijing Engineering and Technology Research Center of Food Additives, Beijing Technology & Business University (BTBU), Beijing 100048, China
3
State Key Laboratory of Bioactive Seaweed Substances, Ministry of Agriculture Key Laboratory of Seaweed Fertilizers, Qingdao Brightmoon Seaweed Group Co Ltd., Qingdao 266400, China
*
Authors to whom correspondence should be addressed.
Antioxidants 2020, 9(2), 127; https://doi.org/10.3390/antiox9020127
Received: 29 December 2019
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Revised: 24 January 2020
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Accepted: 30 January 2020
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Published: 2 February 2020
(This article belongs to the Special Issue Oxidative Stress Modulators and Functional Foods)
After extraction from jujube pomace and purification by two columns (DEAE-Sepharose Fast Flow and Sepharcyl S-300), the structure of SAZMP4 was investigated by HPGPC, GC, FI-IR, GC-MS, NMR, SEM, and AFM. Analysis determined that SAZMP4 (Mw = 28.94 kDa) was a pectic polysaccharide mainly containing 1,4-linked GalA (93.48%) with side chains of 1,2,4-linked Rha and 1,3,5-linked Ara and terminals of 1-linked Rha and 1-linked Ara, which might be the homogalacturonan (HG) type with side chains of the RG-I type, corresponding to the results of NMR. In AFM and SEM images, self-assembly and aggregation of SAZMP4 were respectively observed indicating its structural features. The antioxidant activity of SAZMP4 against H2O2-induced oxidative stress in Caco-2 cells was determined by activity of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) as well as malondialdehyde (MDA) and reactive oxygen species (ROS) levels, indicating SAZMP4 can be a natural antioxidant. Also, a better water retention capacity and thermal stability of SAZMP4 was observed based on DSC analysis, which could be applied in food industry as an additive.
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MDPI and ACS Style
Lin, X.; Liu, K.; Yin, S.; Qin, Y.; Shen, P.; Peng, Q. A Novel Pectic Polysaccharide of Jujube Pomace: Structural Analysis and Intracellular Antioxidant Activities. Antioxidants 2020, 9, 127. https://doi.org/10.3390/antiox9020127
AMA Style
Lin X, Liu K, Yin S, Qin Y, Shen P, Peng Q. A Novel Pectic Polysaccharide of Jujube Pomace: Structural Analysis and Intracellular Antioxidant Activities. Antioxidants. 2020; 9(2):127. https://doi.org/10.3390/antiox9020127
Chicago/Turabian StyleLin, Ximeng, Keshan Liu, Sheng Yin, Yimin Qin, Peili Shen, and Qiang Peng. 2020. "A Novel Pectic Polysaccharide of Jujube Pomace: Structural Analysis and Intracellular Antioxidant Activities" Antioxidants 9, no. 2: 127. https://doi.org/10.3390/antiox9020127
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