The Apoplastic and Symplastic Antioxidant System in Onion: Response to Long-Term Salt Stress
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Material
2.2. Treatments and Experimental Design
2.3. Sample Selection
2.4. Histochemical ROS Detection
2.5. Oxidative Stress Parameters
2.5.1. Electrolyte Leakage Measurement
2.5.2. Lipid Peroxidation
2.6. Apoplast Extraction
2.7. Leaf Enzyme Extraction and Enzymatic Analyses
2.8. Ascorbate and Glutathione Measurements
2.9. Statistical Analysis
3. Results
3.1. Effect of Salt Stress on Oxidative Stress Parameters
3.2. Antioxidant Enzymatic Activities
3.3. Non-Enzymatic Antioxidants
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Genotype | Treatment | ASC | DHA | Redox State | |
---|---|---|---|---|---|
(nmol g−1 FW) | (%) | ||||
‘Texas 502’ | Control | 1020.9 b,c | 523.4 a | 66.1 | |
Leaves | Salinity | 1166.1 a | 536.7 a | 68.7 | |
‘Granex 429’ | Control | 986.2 c | 494.2 a | 66.6 | |
Salinity | 1151.3 a,b | 451.8 a | 71.8 | ||
‘Texas 502’ | Control | 893.5 a,b | 602.3 a | 59.7 | |
Roots | Salinity | 1030.2 a | 462.3 b | 69.0 | |
‘Granex 429’ | Control | 961.8 a,b | 503.3 b | 65.6 | |
Salinity | 808.1 b | 262.5 c | 75.5 |
Genotype | Treatment | GSH | GSSG | Redox State | |
---|---|---|---|---|---|
(nmol g−1 FW) | (%) | ||||
‘Texas 502’ | Control | 11.59 a | 2.66 c | 81.3 | |
Leaves | Salino | 5.84 b | 3.94 b | 59.7 | |
‘Granex 429’ | Control | 6.21 b | 4.42 b | 58.4 | |
Salino | 3.00 b | 5.76 a | 34.2 | ||
‘Texas 502’ | Control | 52.43 a | 9.16 a | 85.1 | |
Roots | Salino | 56.82 a | 9.20 a | 86.1 | |
‘Granex 429’ | Control | 34.97 b | 10.30 a | 77.2 | |
Salino | 48.1 a,b | 11.55 a | 80.6 |
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García, G.; Clemente-Moreno, M.J.; Díaz-Vivancos, P.; García, M.; Hernández, J.A. The Apoplastic and Symplastic Antioxidant System in Onion: Response to Long-Term Salt Stress. Antioxidants 2020, 9, 67. https://doi.org/10.3390/antiox9010067
García G, Clemente-Moreno MJ, Díaz-Vivancos P, García M, Hernández JA. The Apoplastic and Symplastic Antioxidant System in Onion: Response to Long-Term Salt Stress. Antioxidants. 2020; 9(1):67. https://doi.org/10.3390/antiox9010067
Chicago/Turabian StyleGarcía, Grisaly, María José Clemente-Moreno, Pedro Díaz-Vivancos, Marina García, and José Antonio Hernández. 2020. "The Apoplastic and Symplastic Antioxidant System in Onion: Response to Long-Term Salt Stress" Antioxidants 9, no. 1: 67. https://doi.org/10.3390/antiox9010067