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Optimization of Ultrasound-Assisted Extraction of Polyphenols from Myrtus communis L. Pericarp

1
Laboratoire de Biomathématiques, Biophysique, Biochimie, et Scientométrie (L3BS), Faculté des Sciences de la Natureet de la Vie, Université de Bejaia, 06000 Bejaia, Algeria
2
QOPNA & LAQV-REQUIMTE, Department of Chemistry, University of Aveiro, 3810-193 Aveiro, Portugal
3
Faculté des Sciences de la Nature et de la Vie, Université de Jijel, 18000 Jijel, Algeria
*
Authors to whom correspondence should be addressed.
Antioxidants 2019, 8(7), 205; https://doi.org/10.3390/antiox8070205
Received: 17 May 2019 / Revised: 16 June 2019 / Accepted: 26 June 2019 / Published: 2 July 2019
(This article belongs to the Special Issue Antioxidant Activity of Polyphenolic Plant Extracts)
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Abstract

Response surface methodology (RSM) was used to optimize the extraction of phenolics from pericap of Myrtus communis using ultrasound-assisted extraction (UAE). The results were compared with those obtained by microwave-assisted extraction (MAE) and conventional solvent extraction (CSE) methods. The individual compounds of the optimized extract obtained by UAE were identified by ultra-high-performance liquid chromatography coupled with diode array detection and electrospray ionization mass spectrometry (UHPLC-DAD-ESI-MSn). The yield of total phenolic compounds (TPC) was affected more significantly by ethanol concentration, irradiation time, liquid solvent-to-solid ratio (p < 0.0001) and amplitude (p = 0.0421) and optimal parameters conditions set by the RSM model were 70% (v/v), 7.5 min and 30%, respectively. The experimental yield of TPC (241.66 ± 12.77 mg gallic acid equivalent/g dry weight) confirmed the predicted value (235.52 ± 9.9 mg gallic acid equivalent/g dry weight), allowing also to confirm the model validity. Under optimized conditions, UAE was more efficient than MAE and CSE in extracting antioxidants, which comprised mostly myricetin glycosides. Globally, the present work demonstrated that, compared to MAE and CSE, UAE is an efficient method for phenolic extraction from M. communis pericarp, enabling to reduce the working time and the solvent consumption. View Full-Text
Keywords: myrtle; ultrasound-assisted extraction; phenolic compounds; antioxidant capacity; liquid chromatography analysis; mass spectrometry myrtle; ultrasound-assisted extraction; phenolic compounds; antioxidant capacity; liquid chromatography analysis; mass spectrometry
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Bouaoudia-Madi, N.; Boulekbache-Makhlouf, L.; Madani, K.; Silva, A.M.; Dairi, S.; Oukhmanou–Bensidhoum, S.; Cardoso, S.M. Optimization of Ultrasound-Assisted Extraction of Polyphenols from Myrtus communis L. Pericarp. Antioxidants 2019, 8, 205.

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