PON2 Deficiency Leads to Increased Susceptibility to Diet-Induced Obesity
Abstract
:1. Introduction
2. Materials and Methods
2.1. Mice and Diet
2.2. Plasma Lipids, Glucose, and Insulin Assays
2.3. Body Composition by Quantitative Nuclear Magnetic Resonance
2.4. Glucose Tolerance Test
2.5. Metabolic Chamber Study
2.6. Oxygen Consumption Study
2.7. RNA Isolation and Quantitative RT-PCR Analyses
2.8. Determination of Average Adipocyte Size and Adipose Cell Number
2.9. Statistical Analysis
3. Results and Discussion
3.1. PON2-def Mice Are More Susceptible to Diet-Induced Obesity and Exhibit Glucose Intolerance
3.2. PON2-def Mice Exhibit Altered Systemic Energy Balance and a Decreased Oxygen Consumption Rate in the White Adipose Tissue
3.3. Gene Expression Analysis of WAT and BAT
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Genotype | n | Triglyceride | Total Chol. | HDL Chol. | VLDL/IDL/LDL Chol. | Unesterified Chol. | Free Fatty Acid | Glucose | Insulin |
---|---|---|---|---|---|---|---|---|---|
WT | 9 | 35 (7) | 128 (5) | 91 (4) | 47 (12) | 18 (1) | 48 (3) | 143 (10) | 336 (48) |
PON2KO | 10 | 34 (8) | 118 (17) | 87 (13) | 30 (6) | 19 (2) | 47 (3) | 147 (8) | 485 (47) |
p value | 0.91 | 0.58 | 0.80 | 0.20 | 0.63 | 0.76 | 0.71 | 0.04 |
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Shih, D.M.; Meng, Y.; Sallam, T.; Vergnes, L.; Shu, M.L.; Reue, K.; Tontonoz, P.; Fogelman, A.M.; Lusis, A.J.; Reddy, S.T. PON2 Deficiency Leads to Increased Susceptibility to Diet-Induced Obesity. Antioxidants 2019, 8, 19. https://doi.org/10.3390/antiox8010019
Shih DM, Meng Y, Sallam T, Vergnes L, Shu ML, Reue K, Tontonoz P, Fogelman AM, Lusis AJ, Reddy ST. PON2 Deficiency Leads to Increased Susceptibility to Diet-Induced Obesity. Antioxidants. 2019; 8(1):19. https://doi.org/10.3390/antiox8010019
Chicago/Turabian StyleShih, Diana M., Yonghong Meng, Tamer Sallam, Laurent Vergnes, Michelle L. Shu, Karen Reue, Peter Tontonoz, Alan M. Fogelman, Aldons J. Lusis, and Srinivasa T. Reddy. 2019. "PON2 Deficiency Leads to Increased Susceptibility to Diet-Induced Obesity" Antioxidants 8, no. 1: 19. https://doi.org/10.3390/antiox8010019
APA StyleShih, D. M., Meng, Y., Sallam, T., Vergnes, L., Shu, M. L., Reue, K., Tontonoz, P., Fogelman, A. M., Lusis, A. J., & Reddy, S. T. (2019). PON2 Deficiency Leads to Increased Susceptibility to Diet-Induced Obesity. Antioxidants, 8(1), 19. https://doi.org/10.3390/antiox8010019