Next Article in Journal
Oxidative Stress in the Brain: Basic Concepts and Treatment Strategies in Stroke
Next Article in Special Issue
Peroxiredoxin 6 Applied after Exposure Attenuates Damaging Effects of X-ray Radiation in 3T3 Mouse Fibroblasts
Previous Article in Journal
Autophagy Is Involved in the Viability of Overexpressing Thioredoxin o1 Tobacco BY-2 Cells under Oxidative Conditions
Previous Article in Special Issue
Planarians as an In Vivo Experimental Model for the Study of New Radioprotective Substances
Article

Radioprotective and Radiomitigative Effects of Melatonin in Tissues with Different Proliferative Activity

Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, 142290 Moscow Region, Russia
*
Author to whom correspondence should be addressed.
Academic Editors: Elena Obrador Pla and Alegria Montoro
Antioxidants 2021, 10(12), 1885; https://doi.org/10.3390/antiox10121885
Received: 8 October 2021 / Revised: 18 November 2021 / Accepted: 24 November 2021 / Published: 25 November 2021
We used various markers to analyze damage to mouse tissues (spleen and cerebral cortex) which have different proliferative activity and sensitivity to ionizing radiation (IR). We also assessed the degree of modulation of damages that occurs when melatonin is administered to mice prior to and after their X-ray irradiation. The data from this study showed that lesions in nuclear DNA (nDNA) were repaired more actively in the spleen than in the cerebral cortex of mice irradiated and treated with melatonin (N-acetyl-5-methoxytryptamine). Mitochondrial biogenesis involving mitochondrial DNA (mtDNA) synthesis was activated in both tissues of irradiated mice. A significant proportion of the newly synthesized mtDNA molecules were mutant copies that increase oxidative stress. Melatonin reduced the number of mutant mtDNA copies and the level of H2O2 in both tissues of the irradiated mice. Melatonin promoted the restoration of ATP levels in the tissues of irradiated mice. In the mouse tissues after exposure to X-ray, the level of malondialdehyde (MDA) increased and melatonin was able to reduce it. The MDA concentration was higher in the cerebral cortex tissue than that in the spleen tissue of the mouse. In mouse tissues following irradiation, the glutathione (GSH) level was low. The spleen GSH content was more than twice as low as that in the cerebral cortex. Melatonin helped restore the GSH levels in the mouse tissues. Although the spleen and cerebral cortex tissues of mice differ in the baseline values of the analyzed markers, the radioprotective and radiomitigative potential of melatonin was observed in both tissues. View Full-Text
Keywords: radiation; melatonin; nDNA-repair; mtDNA-mutations; oxidation stress; protection; mitigation; H2O2; ATP; MDA; GSH radiation; melatonin; nDNA-repair; mtDNA-mutations; oxidation stress; protection; mitigation; H2O2; ATP; MDA; GSH
Show Figures

Figure 1

MDPI and ACS Style

Abdullaev, S.A.; Glukhov, S.I.; Gaziev, A.I. Radioprotective and Radiomitigative Effects of Melatonin in Tissues with Different Proliferative Activity. Antioxidants 2021, 10, 1885. https://doi.org/10.3390/antiox10121885

AMA Style

Abdullaev SA, Glukhov SI, Gaziev AI. Radioprotective and Radiomitigative Effects of Melatonin in Tissues with Different Proliferative Activity. Antioxidants. 2021; 10(12):1885. https://doi.org/10.3390/antiox10121885

Chicago/Turabian Style

Abdullaev, Serazhutdin A., Sergey I. Glukhov, and Azhub I. Gaziev. 2021. "Radioprotective and Radiomitigative Effects of Melatonin in Tissues with Different Proliferative Activity" Antioxidants 10, no. 12: 1885. https://doi.org/10.3390/antiox10121885

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop