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Arcopilus aureus MaC7A as a New Source of Resveratrol: Assessment of Amino Acid Precursors, Volatiles, and Fungal Enzymes for Boosting Resveratrol Production in Batch Cultures

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CONACyT-Centro Universitario de Vinculación y Transferencia de Tecnología-DITCo, Benemérita Universidad Autónoma de Puebla, Cd. Universitaria, Puebla 72570, Mexico
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Centro de Agroecología, Instituto de Ciencias, Benemérita Universidad Autónoma de Puebla, Edificio VAL 1, Km 1,7 Carretera a San Baltazar Tetela, San Pedro Zacachimalpa, Puebla 72960, Mexico
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Centro Universitario de Vinculación y Transferencia de Tecnología, Benemérita Universidad Autónoma de Puebla, DITCo, Cd. Universitaria, Puebla 72570, Mexico
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Centro de Investigaciones en Ciencias Microbiológicas, Instituto de Ciencias, Benemérita Universidad Autónoma de Puebla, Ciudad Universitaria, Puebla 72570, Mexico
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Author to whom correspondence should be addressed.
Academic Editor: Antonio Valero
Appl. Sci. 2021, 11(10), 4583; https://doi.org/10.3390/app11104583
Received: 23 April 2021 / Revised: 12 May 2021 / Accepted: 14 May 2021 / Published: 18 May 2021
(This article belongs to the Section Applied Biosciences and Bioengineering)
The chemical factors that regulate the synthesis of resveratrol (RV) in filamentous fungi are still unknown. This work reports on the RV production by Arcopilus aureus MaC7A under controlled conditions and the effect of amino acid precursors (PHE and TYR), monoterpenes (limonone, camphor, citral, thymol, menthol), and mixtures of hydrolytic enzymes (Glucanex) as elicitors for boosting fungal RV. Batch cultures with variable concentrations of PHE and TYR (50–500 mg L−1) stimulated RV production from 127.9 ± 4.6 to 221.8 ± 5.2 mg L−1 in basic cultures developed in PDB (pH 7) added with 10 g L−1 peptone at 30 °C. Maximum levels of RV and biomass were maintained during days 6–8 under these conditions, whereas a dramatic RV decrease was observed from days 10–12 without any loss of biomass. Among the tested volatiles, citral (50 mg L−1) enhanced RV production until 187.8 ± 2.2 mg L−1 in basic cultures, but better results were obtained with Glucanex (100 mg L−1; 198.3 ± 7.6 mg L−1 RV). Optimized batch cultures containing TYR (200 mg L−1), citral (50 mg L−1), thymol (50 mg L−1), and Glucanex (100 mg L−1) produced up to 237.6 ± 4.7 mg L−1 of RV. Our results suggest that low concentrations of volatiles and mixtures of isoenzymes with β-1, 3 glucanase activity increase the biosynthesis of fungal RV produced by A. aureus MaC7A in batch cultures. View Full-Text
Keywords: Arcopilus aureus; resveatrol; batch cultures; elicitors Arcopilus aureus; resveatrol; batch cultures; elicitors
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MDPI and ACS Style

Villa-Ruano, N.; Morales-Mora, L.Á.; Varela-Caselis, J.L.; Rivera, A.; Valencia de Ita, M.d.l.Á.; Romero-Arenas, O. Arcopilus aureus MaC7A as a New Source of Resveratrol: Assessment of Amino Acid Precursors, Volatiles, and Fungal Enzymes for Boosting Resveratrol Production in Batch Cultures. Appl. Sci. 2021, 11, 4583. https://doi.org/10.3390/app11104583

AMA Style

Villa-Ruano N, Morales-Mora LÁ, Varela-Caselis JL, Rivera A, Valencia de Ita MdlÁ, Romero-Arenas O. Arcopilus aureus MaC7A as a New Source of Resveratrol: Assessment of Amino Acid Precursors, Volatiles, and Fungal Enzymes for Boosting Resveratrol Production in Batch Cultures. Applied Sciences. 2021; 11(10):4583. https://doi.org/10.3390/app11104583

Chicago/Turabian Style

Villa-Ruano, Nemesio, Luis Á. Morales-Mora, Jenaro L. Varela-Caselis, Antonio Rivera, María d.l.Á. Valencia de Ita, and Omar Romero-Arenas. 2021. "Arcopilus aureus MaC7A as a New Source of Resveratrol: Assessment of Amino Acid Precursors, Volatiles, and Fungal Enzymes for Boosting Resveratrol Production in Batch Cultures" Applied Sciences 11, no. 10: 4583. https://doi.org/10.3390/app11104583

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