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3 January 2026

Weighted Single-Step Genome-Wide Association Study Identifies Candidate Genes for Carcass Traits and Primal Cut Yields in Hanwoo Cattle

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1
Dasan Pig Breeding Co., Namwon 55716, Republic of Korea
2
Department of Animal Biotechnology, Jeonbuk National University, Jeonju 54896, Republic of Korea
3
Animal Genetic Resources Research Center, National Institute of Animal Science, Rural Development Administration, 224, Hamyang 50000, Republic of Korea
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Authors to whom correspondence should be addressed.
This article belongs to the Special Issue Advances in Cattle Genetics and Breeding

Simple Summary

The economic value of Hanwoo carcasses is determined not only by marbling score but also by primal cut yields. However, the genetic basis of primal cut yields remains largely unknown. This study identified genomic regions associated with carcass traits and primal cut yields. For carcass traits, candidate genes were identified for carcass weight (XKR4, COBL), eye muscle area (LCORL, TGFBR2), backfat thickness (ATG7, MYPN), and marbling score (TWIST2, BMP4). For primal cut yields, the chromosome 6 region containing WDR1 was commonly identified across six traits and the chromosome 4 region containing CACNA2D1 across five traits, suggesting pleiotropic effects. These findings provide candidate genomic regions that may inform future breeding strategies aimed at improving both carcass quality and meat yield, pending validation in independent populations.

Abstract

This study identified genomic regions associated with carcass traits and primal cut yields in Hanwoo cattle using weighted single-step genome-wide association study (WssGWAS). A total of 50,227 carcass records and genomic data from 23,573 animals with 45,057 single-nucleotide polymorphisms were analyzed. Heritability estimates were 0.24 for carcass weight, 0.22 for eye muscle area, 0.31 for backfat thickness, and 0.36 for marbling score, while those for primal cut yields ranged from 0.02 to 0.26. For carcass traits, candidate genes were identified for carcass weight (XKR4 2.35%, COBL 1.26%), eye muscle area (LCORL 1.56%, TGFBR2 1.49%), backfat thickness (ATG7 1.27%, MYPN 1.33%), and marbling score (TWIST2 1.16%, BMP4 1.14%). For primal cut yields, the chromosome 6 region containing WDR1 was commonly identified across six traits and the chromosome 4 region containing CACNA2D1 across five traits; the chromosome 28 region containing SIRT1 explained the highest genetic variance (6.46%) for striploin. These pleiotropic regions are potential targets for genomic selection to improve production efficiency and carcass value in Hanwoo.

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