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Article

Gut Digestive Function and Microbiome after Correction of Experimental Dysbiosis in Rats by Indigenous Bifidobacteria

1
Pavlov Institute of Physiology, Russian Academy of Sciences, 199034 Saint-Petersburg, Russia
2
Department of Molecular Microbiology, Institute of Experimental Medicine, 197376 Saint-Petersburg, Russia
3
Department of Medical Microbiology, North-Western State Medical University Named after I.I. Mechnikov, Ministry of Health of the Russian Federation, 195067 Saint-Petersburg, Russia
4
Department of Fundamental Problems of Medicine and Medical Technologies, Saint-Petersburg State University, 199034 Saint-Petersburg, Russia
*
Author to whom correspondence should be addressed.
Academic Editor: Serguei Fetissov
Microorganisms 2021, 9(3), 522; https://doi.org/10.3390/microorganisms9030522
Received: 1 February 2021 / Revised: 25 February 2021 / Accepted: 26 February 2021 / Published: 4 March 2021
(This article belongs to the Special Issue Gut Microbiota-Brain Axis in Regulation of Feeding Behavior)
In recent years, great interest has arisen in the use of autoprobiotics (indigenous bacteria isolated from the organism and introduced into the same organism after growing). This study aimed to evaluate the effects of indigenous bifidobacteria on intestinal microbiota and digestive enzymes in a rat model of antibiotic-associated dysbiosis. Our results showed that indigenous bifidobacteria (the Bf group) accelerate the disappearance of dyspeptic symptoms in rats and prevent an increase in chyme mass in the upper intestine compared to the group without autoprobiotics (the C1 group), but significantly increase the mass of chyme in the colon compared to the C1 group and the control group (healthy animals). In the Bf group in the gut microbiota, the content of opportunistic bacteria (Proteus spp., enteropathogenic Escherichia coli) decreased, and the content of some beneficial bacteria (Bifidobacterium spp., Dorea spp., Blautia spp., the genus Ruminococcus, Prevotella, Oscillospira) changed compared to the control group. Unlike the C1 group, in the Bf group there was no decrease in the specific activities of maltase and alkaline phosphatase in the mucosa of the upper intestine, but the specific activity of maltase was decreased in the colon chyme compared to the control and C1 groups. In the Bf group, the specific activity of aminopeptidase N was reduced in the duodenum mucosa and the colon chyme compared to the control group. We concluded that indigenous bifidobacteria can protect the microbiota and intestinal digestive enzymes in the intestine from disorders caused by dysbiosis; however, there may be impaired motor function of the colon. View Full-Text
Keywords: autoprobiotics; intestinal digestive enzymes; microbiome autoprobiotics; intestinal digestive enzymes; microbiome
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MDPI and ACS Style

Gromova, L.V.; Ermolenko, E.I.; Sepp, A.L.; Dmitrieva, Y.V.; Alekseeva, A.S.; Lavrenova, N.S.; Kotyleva, M.P.; Kramskaya, T.A.; Karaseva, A.B.; Suvorov, A.N.; Gruzdkov, A.A. Gut Digestive Function and Microbiome after Correction of Experimental Dysbiosis in Rats by Indigenous Bifidobacteria. Microorganisms 2021, 9, 522. https://doi.org/10.3390/microorganisms9030522

AMA Style

Gromova LV, Ermolenko EI, Sepp AL, Dmitrieva YV, Alekseeva AS, Lavrenova NS, Kotyleva MP, Kramskaya TA, Karaseva AB, Suvorov AN, Gruzdkov AA. Gut Digestive Function and Microbiome after Correction of Experimental Dysbiosis in Rats by Indigenous Bifidobacteria. Microorganisms. 2021; 9(3):522. https://doi.org/10.3390/microorganisms9030522

Chicago/Turabian Style

Gromova, Lyudmila V., Elena I. Ermolenko, Anastasiya L. Sepp, Yulia V. Dmitrieva, Anna S. Alekseeva, Nadezhda S. Lavrenova, Mariya P. Kotyleva, Tatyana A. Kramskaya, Alena B. Karaseva, Alexandr N. Suvorov, and Andrey A. Gruzdkov 2021. "Gut Digestive Function and Microbiome after Correction of Experimental Dysbiosis in Rats by Indigenous Bifidobacteria" Microorganisms 9, no. 3: 522. https://doi.org/10.3390/microorganisms9030522

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