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Open AccessArticle

Analytical Performances of the Panther Fusion System for the Detection of Respiratory Viruses in the French National Reference Centre of Lyon, France

1
Laboratoire de Bactériologie-Hygiène, CHU Poitiers, Département des Agents Infectieux, 86021 Poitiers, France
2
Laboratoire de Virologie, Hospices Civils de Lyon, Institut des Agents Infectieux, Centre National de Référence des Virus des Infections Respiratoires (dont la Grippe), Hôpital de la Croix-Rousse, 103 grande rue de la Croix-Rousse, CEDEX 04, 69317 Lyon, France
3
Laboratoire de Virologie, Hospices Civils de Lyon, Institut des Agents Infectieux, Centre National de Référence des Enterovirus et Paréchovirus, Hôpital de la Croix-Rousse, 103 grande rue de la Croix-Rousse, CEDEX 04, 69317 Lyon, France
4
Laboratoire de Virologie, Hospices Civils de Lyon, Institut des Agents Infectieux, Hôpital de la Croix-Rousse, 103 grande rue de la Croix-Rousse, CEDEX 04, 69317 Lyon, France
*
Authors to whom correspondence should be addressed.
Microorganisms 2020, 8(9), 1371; https://doi.org/10.3390/microorganisms8091371
Received: 20 July 2020 / Revised: 1 September 2020 / Accepted: 3 September 2020 / Published: 7 September 2020
Respiratory infection are mainly caused by viral pathogens. During the 2017–2018 epidemic season, Panther Fusion® Respiratory kits (Influenza virus A&B (FluA&B), respiratory syncytial virus (RSV), adenovirus (ADV), metapneumovirus (MPV), rhinovirus (RV), parainfluenzae virus (PIV), were compared to the Respiratory MultiWells System r-gene. Respiratory clinical specimens were tested retrospectively (n = 268) and prospectively (n = 463). Analytical performances were determined (sensitivity –Sep-, specificity –Spe- and κ) considering concordances of ≥2 molecular testing specific to each viral target (discrepant results were verified at the National Reference Centres for Enteroviruses or Respiratory viruses, Lyon, France). After retrospective (and prospective) testing, Sep, Spe, and κ were 100% (97.7%), 100% (99%) and 100% (94%) for FluA: 100% (95.5%), 100% (99.3%) and 100% (94%) for FluB, and 100% (88.5%), 100% (98.7%) and 100% (89%) for RSV; 82.1% (41.7%), 100% (99.5%) and 86% (54%) for ADV; 94.7% (73.7%), 96.1% (98.0%) and 91% (65%) for MPV; 96.1% (94.6%), 90.2% (98.5%) and 86% (91%) for HRV; and 90% (72.7%), 100% (99.3%) and 91% (72%), respectively, for PIV. Analytical performances were above 85% for all viruses except for ADV, MPV and PIV, confirming the analytical performance of the Panther Fusion system, a high throughput system with reduced turn-around-time, when compared to non-automated systems. View Full-Text
Keywords: respiratory viruses; influenza; diagnostic PCR; performance evaluation; automation; respiratory tract samples; sample-to-answer real-time PCR respiratory viruses; influenza; diagnostic PCR; performance evaluation; automation; respiratory tract samples; sample-to-answer real-time PCR
MDPI and ACS Style

Pichon, M.; Valette, M.; Schuffenecker, I.; Billaud, G.; Lina, B. Analytical Performances of the Panther Fusion System for the Detection of Respiratory Viruses in the French National Reference Centre of Lyon, France. Microorganisms 2020, 8, 1371.

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