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Article

Characterizing the Fungal Microbiome in Date (Phoenix dactylifera) Fruit Pulp and Peel from Early Development to Harvest

1
Department of Agricultural, Forestry and Food Sciences (DISAFA), University of Turin, 10095 Grugliasco, TO, Italy
2
AGROINNOVA—Centre of Competence for the Innovation in the Agro-environmental Sector, University of Turin, 10095 Grugliasco, TO, Italy
3
Institute of Environmental Biotechnology, Graz University of Technology, 8010 Graz, Austria
4
Department of Ecology, Environment and Plant Sciences, Stockholm University, 10691 Stockholm, Sweden
5
Department of Postharvest Science, Agricultural Research Organization (ARO), The Volcani Center, Bet Dagan 5020000, Israel
6
U.S. Department of Agriculture—Agricultural Research Service (USDA-ARS), Kearneysville, WV 25430, USA
*
Author to whom correspondence should be addressed.
Microorganisms 2020, 8(5), 641; https://doi.org/10.3390/microorganisms8050641
Received: 2 April 2020 / Revised: 21 April 2020 / Accepted: 24 April 2020 / Published: 28 April 2020
(This article belongs to the Special Issue Applying Metaorganism Studies to the Fruit Microbiome: A New Frontier)
Date palm (Phoenix dactylifera) is considered to be a highly important food crop in several African and Middle Eastern countries due to its nutritional value and health-promoting properties. Microbial contamination of dates has been of concern to consumers, but very few works have analyzed in detail the microbial load of the different parts of date fruit. In the present work, we characterized the fungal communities of date fruit using a metagenomic approach, analyzing the data for differences between microbial populations residing in the pulp and peel of “Medjool” dates at the different stages of fruit development. The results revealed that Penicillium, Cladosporium, Aspergillus, and Alternaria were the most abundant genera in both parts of the fruit, however, the distribution of taxa among the time points and tissue types (peel vs. pulp) was very diverse. Penicillium was more abundant in the pulp at the green developmental stage (Kimri), while Aspergillus was more frequent in the peel at the brown developmental stage (Tamer). The highest abundance of Alternaria was detected at the earliest sampled stage of fruit development (Hababauk stage). Cladosporium had a high level of abundance in peel tissues at the Hababauk and yellow (Khalal) stages. Regarding the yeast community, the abundance of Candida remained stable up until the Khalal stage, but exhibited a dramatic increase in abundance at the Tamer stage in peel tissues, while the level of Metschnikowia, a genus containing several species with postharvest biocontrol activity, exhibited no significant differences between the two tissue types or stages of fruit development. This work constitutes a comprehensive metagenomic analysis of the fungal microbiome of date fruits, and has identified changes in the composition of the fungal microbiome in peel and pulp tissues at the different stages of fruit development. Notably, this study has also characterized the endophytic fungal microbiome present in pulp tissues of dates. View Full-Text
Keywords: date; Phoenix dactylifera; microbiome; metagenome; ITS; post-harvest date; Phoenix dactylifera; microbiome; metagenome; ITS; post-harvest
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MDPI and ACS Style

Piombo, E.; Abdelfattah, A.; Danino, Y.; Salim, S.; Feygenberg, O.; Spadaro, D.; Wisniewski, M.; Droby, S. Characterizing the Fungal Microbiome in Date (Phoenix dactylifera) Fruit Pulp and Peel from Early Development to Harvest. Microorganisms 2020, 8, 641. https://doi.org/10.3390/microorganisms8050641

AMA Style

Piombo E, Abdelfattah A, Danino Y, Salim S, Feygenberg O, Spadaro D, Wisniewski M, Droby S. Characterizing the Fungal Microbiome in Date (Phoenix dactylifera) Fruit Pulp and Peel from Early Development to Harvest. Microorganisms. 2020; 8(5):641. https://doi.org/10.3390/microorganisms8050641

Chicago/Turabian Style

Piombo, Edoardo; Abdelfattah, Ahmed; Danino, Yaara; Salim, Shoshana; Feygenberg, Oleg; Spadaro, Davide; Wisniewski, Michael; Droby, Samir. 2020. "Characterizing the Fungal Microbiome in Date (Phoenix dactylifera) Fruit Pulp and Peel from Early Development to Harvest" Microorganisms 8, no. 5: 641. https://doi.org/10.3390/microorganisms8050641

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