Next Article in Journal
Grease Waste as a Reservoir of Lipase-Producing Yeast and Description of Limtongella siamensis gen. nov., sp. nov
Next Article in Special Issue
Isolation and Characterization of Genes Responsible for Naphthalene Degradation from Thermophilic Naphthalene Degrader, Geobacillus sp. JF8
Previous Article in Journal
Mutation and Recombination Rates Vary Across Bacterial Chromosome
Previous Article in Special Issue
Erratum: Farber, R.; Rosenberg, A.; Rozenfeld, S.; Banet, G.; Cahan, R. Bioremediation of Artificial Diesel-Contaminated Soil Using Bacterial Consortium Immobilized to Plasma-Pretreated Wood Waste. Microorganisms 2019, 7, 497
Open AccessArticle

Biotransformation of Monocyclic Phenolic Compounds by Bacillus licheniformis TAB7

1
Biotechnology Research Center, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan
2
Collaborative Research Institute for Innovative Microbiology, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan
3
Agriculture and Biotechnology Business Division, Toyota Motor Corporation, 1099 Marune, Kurozasa-cho, Miyoshi-shi, Aichi 470-0201, Japan
*
Author to whom correspondence should be addressed.
Current address: Institute for Tuberculosis Research, University of Illinois at Chicago, 833 South Wood Street, Chicago, IL 60612, USA.
Microorganisms 2020, 8(1), 26; https://doi.org/10.3390/microorganisms8010026
Received: 29 November 2019 / Revised: 18 December 2019 / Accepted: 19 December 2019 / Published: 21 December 2019
(This article belongs to the Special Issue Microbial Degradation of Xenobiotics)
Bacillus licheniformis strain TAB7 is a bacterium used as a commercial deodorizing agent for compost in Japan. In this work, its ability to biotransform the following monocyclic phenolic compounds was assessed: ferulate, vanillate, p-coumarate, caffeate, protocatechuate, syringate, vanillin, and cinnamate (a precursor for some phenolic compounds). These compounds are abundant in composting material and are reported to have allelopathic properties. They come from sources such as plant material decomposition or agro-industrial waste. Biotransformation assays were carried out in LB supplemented with 0.2 mg/mL of an individual phenolic compound and incubated for up to 15 days followed by extraction and HPLC analysis. The results showed that TAB7 could biotransform ferulate, caffeate, p-coumarate, vanillate, protocatechuate, and vanillin. It, however, had a poor ability to transform cinnamate and syringate. LC-MS/MS analysis showed that ferulate was transformed into 4-vinylguaiacol as the final product, while caffeate was transformed into 4-ethylcatechol. TAB7 genome analysis suggested that, while TAB7 may not mineralize phenolic compounds, it harbored genes possibly encoding phenolic acid decarboxylase, vanillate decarboxylase, and some protocatechuate degradation pathway enzymes, which are involved in the catabolism of phenolic compounds known to have negative allelopathy on some plants. The results thus suggested that TAB7 can reduce such phenolic compounds in compost. View Full-Text
Keywords: agro-industrial wastes; allelochemicals; Bacillus licheniformis; biotransformation; phenolic compounds agro-industrial wastes; allelochemicals; Bacillus licheniformis; biotransformation; phenolic compounds
Show Figures

Figure 1

MDPI and ACS Style

Mpofu, E.; Chakraborty, J.; Suzuki-Minakuchi, C.; Okada, K.; Kimura, T.; Nojiri, H. Biotransformation of Monocyclic Phenolic Compounds by Bacillus licheniformis TAB7. Microorganisms 2020, 8, 26. https://doi.org/10.3390/microorganisms8010026

AMA Style

Mpofu E, Chakraborty J, Suzuki-Minakuchi C, Okada K, Kimura T, Nojiri H. Biotransformation of Monocyclic Phenolic Compounds by Bacillus licheniformis TAB7. Microorganisms. 2020; 8(1):26. https://doi.org/10.3390/microorganisms8010026

Chicago/Turabian Style

Mpofu, Enock; Chakraborty, Joydeep; Suzuki-Minakuchi, Chiho; Okada, Kazunori; Kimura, Toshiaki; Nojiri, Hideaki. 2020. "Biotransformation of Monocyclic Phenolic Compounds by Bacillus licheniformis TAB7" Microorganisms 8, no. 1: 26. https://doi.org/10.3390/microorganisms8010026

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop