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Article

Identification and Functional Analysis of NLP-Encoding Genes from the Postharvest Pathogen Penicillium expansum

1
Department of Postharvest Science, Agricultural Research Organization, the Volcani Center, Rishon LeZion 7505101, Israel
2
Appalachian Fruit Research Station, Agricultural Research Service, United States Department of Agriculture, Wiltshire Road, Kearneysville, WV 25430, USA
3
Instituto de Agroquímica y Tecnología de Alimentos (IATA-CSIC), Calle Catedrático Agustin Escardino 7, Paterna 46980, Valencia 46980, Spain
4
Department of Plant Pathology and Weed Research, ARO, the Volcani Center, Newe-Yaar Research Center, Ramat Yeshai 30095, Israel
*
Author to whom correspondence should be addressed.
Microorganisms 2019, 7(6), 175; https://doi.org/10.3390/microorganisms7060175
Received: 21 May 2019 / Revised: 3 June 2019 / Accepted: 13 June 2019 / Published: 15 June 2019
(This article belongs to the Special Issue Plant Microbial Interactions)
Penicillium expansum is a major postharvest pathogen that infects different fruits, mainly through injuries inflicted during harvest or subsequent handling after harvest. Several effectors were suggested to mediate pathogenicity of P. expansum in fruit tissue. Among these effectors Nep1-like proteins (NLPs), produced by various microorganisms with different lifestyles, are known for their ability to induce necrosis in dicot plants and were shown to be involved in virulence of several plant-related pathogens. This study was aimed at the identification and functional characterization of two NLP genes found in the genome of P. expansum. The genes were designated Penlp1 and Penlp2 and were found to code type1 and type3 NLP respectively. Necrosis-inducing activity of the two proteins was demonstrated by transient expression in Nicotiana benthamiana leaves. While Penlp1 expression was induced during apple infection and in liquid culture, the highest level of Penlp2 expression was found in ungerminated spores. Deletion of Penlp1, but not Penlp2, resulted in reduced virulence on apples manifested by reduced rate of lesion development (disease severity). View Full-Text
Keywords: blue mold; apple; Nep1; virulence; pathogenicity; effectors blue mold; apple; Nep1; virulence; pathogenicity; effectors
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MDPI and ACS Style

Levin, E.; Raphael, G.; Ma, J.; Ballester, A.-R.; Feygenberg, O.; Norelli, J.; Aly, R.; Gonzalez-Candelas, L.; Wisniewski, M.; Droby, S. Identification and Functional Analysis of NLP-Encoding Genes from the Postharvest Pathogen Penicillium expansum. Microorganisms 2019, 7, 175. https://doi.org/10.3390/microorganisms7060175

AMA Style

Levin E, Raphael G, Ma J, Ballester A-R, Feygenberg O, Norelli J, Aly R, Gonzalez-Candelas L, Wisniewski M, Droby S. Identification and Functional Analysis of NLP-Encoding Genes from the Postharvest Pathogen Penicillium expansum. Microorganisms. 2019; 7(6):175. https://doi.org/10.3390/microorganisms7060175

Chicago/Turabian Style

Levin, Elena, Ginat Raphael, Jing Ma, Ana-Rosa Ballester, Oleg Feygenberg, John Norelli, Radi Aly, Luis Gonzalez-Candelas, Michael Wisniewski, and Samir Droby. 2019. "Identification and Functional Analysis of NLP-Encoding Genes from the Postharvest Pathogen Penicillium expansum" Microorganisms 7, no. 6: 175. https://doi.org/10.3390/microorganisms7060175

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