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MALDI-TOF Mass Spectrometry and Specific Biomarkers: Potential New Key for Swift Identification of Antimicrobial Resistance in Foodborne Pathogens

1
Luxembourg Institute of Science and Technology (LIST), Environmental Research and Innovation (ERIN) Department, 41 rue du Brill, 4422 Belvaux, Luxembourg
2
Faculté des Sciences, de la Technologie et de la Communication (FSTC), Doctoral School in Science and Engineering (DSSE), University of Luxembourg, 2 avenue de l’Université, 4365 Esch-sur-Alzette, Luxembourg
*
Authors to whom correspondence should be addressed.
Microorganisms 2019, 7(12), 593; https://doi.org/10.3390/microorganisms7120593
Received: 22 October 2019 / Revised: 18 November 2019 / Accepted: 19 November 2019 / Published: 21 November 2019
(This article belongs to the Special Issue Mass Spectrometry: An Undeniable Tool in Current Microbiology)
Matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) is today the reference method for direct identification of microorganisms in diagnostic laboratories, as it is notably time- and cost-efficient. In the context of increasing cases of enteric diseases with emerging multi-drug resistance patterns, there is an urgent need to adopt an efficient workflow to characterize antimicrobial resistance (AMR). Current approaches, such as antibiograms, are time-consuming and directly impact the “patient-physician” workflow. Through this mini-review, we summarize how the detection of specific patterns by MALDI-TOF MS, as well as bioinformatics, become more and more essential in research, and how these approaches will help diagnostics in the future. Along the same lines, the idea to export more precise biomarker identification steps by MALDI-TOF(/TOF) MS data towards AMR identification pipelines is discussed. The study also critically points out that there is currently still a lack of research data and knowledge on different foodborne pathogens as well as several antibiotics families such as macrolides and quinolones, and many questions are still remaining. Finally, the innovative combination of whole-genome sequencing and MALDI-TOF MS could be soon the future for diagnosis of antimicrobial resistance in foodborne pathogens. View Full-Text
Keywords: MALDI-TOF MS; biomarkers; foodborne pathogens; antimicrobial resistance; diagnostics MALDI-TOF MS; biomarkers; foodborne pathogens; antimicrobial resistance; diagnostics
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MDPI and ACS Style

Feucherolles, M.; Cauchie, H.-M.; Penny, C. MALDI-TOF Mass Spectrometry and Specific Biomarkers: Potential New Key for Swift Identification of Antimicrobial Resistance in Foodborne Pathogens. Microorganisms 2019, 7, 593.

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