Next Article in Journal
Coronavirus Disease 2019 (COVID–19): A Short Review on Hematological Manifestations
Previous Article in Journal
Patterns of Ecological Adaptation of Aedes aegypti and Aedes albopictus and Stegomyia Indices Highlight the Potential Risk of Arbovirus Transmission in Yaoundé, the Capital City of Cameroon
Previous Article in Special Issue
Microbiological and Histological Analysis for the Evaluation of Farmed Mussels (Mytilus galloprovincialis) Health Status, in Coastal Areas of Italy
Open AccessArticle

Histopathological and Molecular Study of Pacific Oyster Tissues Provides Insights into V. aestuarianus Infection Related to Oyster Mortality

1
Fish Diseases and Aquaculture Center, IZS of Oristano, 09170 Oristano, Italy
2
Department of Veterinary Medicine, University of Sassari, 07100 Sassari, Italy
3
Mediterranean Center for Disease Control (MCDC), University of Sassari, 07100 Sassari, Italy
*
Authors to whom correspondence should be addressed.
Pathogens 2020, 9(6), 492; https://doi.org/10.3390/pathogens9060492
Received: 26 May 2020 / Revised: 15 June 2020 / Accepted: 18 June 2020 / Published: 20 June 2020
(This article belongs to the Special Issue Spontaneous Diseases of Mollusks)
Consumer preference for healthy and sustainable food products has been steadily increasing in recent years. Bivalve mollusks satisfy these characteristics and have captured ever-increasing market shares. However, the expansion of molluscan culture in worldwide and global trade have favored the spread of pathogens around the world. Combined with environmental changes and intensive production systems this has contributed to the occurrence of mass mortality episodes, thus posing a threat to the production of different species, including the Pacific oyster Crassotrea gigas. In the San Teodoro lagoon, one of the most devoted lagoons to extensive Pacific oyster aquaculture in Sardinia, a mortality outbreak was observed with an estimated 80% final loss of animal production. A study combining cultural, biomolecular and histopathological methods was conducted: (1) to investigate the presence of different Vibrio species and OsHV-1 in selected oyster tissues (digestive gland, gills, and mantle); (2) to quantify Vibrio aestuarianus and to evaluate the severity of hemocyte infiltration in infected tissues; (3) to produce post-amplification data and evaluating ToxR gene as a target for phylogenetic analyses. Results provide new insights into V. aestuarianus infection related to oyster mortality outbreaks and pave the way to the development of tools for oyster management. View Full-Text
Keywords: Crassostrea gigas; aquaculture; real time PCR; histology; Sardinia; bacteria; mass mortality Crassostrea gigas; aquaculture; real time PCR; histology; Sardinia; bacteria; mass mortality
Show Figures

Figure 1

MDPI and ACS Style

Mandas, D.; Salati, F.; Polinas, M.; Sanna, M.A.; Zobba, R.; Burrai, G.P.; Alberti, A.; Antuofermo, E. Histopathological and Molecular Study of Pacific Oyster Tissues Provides Insights into V. aestuarianus Infection Related to Oyster Mortality. Pathogens 2020, 9, 492.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop