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Genetic Diversity of Babesia bovis MSA-1, MSA-2b and MSA-2c in China

1
State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Xujiaping 1, Lanzhou 730046, China
2
Jiangsu Co-Innovation Center for the Prevention and Control of Important Animal Infectious Disease and Zoonose, Yangzhou University, Yangzhou 225009, China
*
Authors to whom correspondence should be addressed.
Pathogens 2020, 9(6), 473; https://doi.org/10.3390/pathogens9060473
Received: 6 April 2020 / Revised: 9 June 2020 / Accepted: 12 June 2020 / Published: 15 June 2020
(This article belongs to the Special Issue Bovine Babesiosis)
The apicomplexan parasite Babesia bovis is a tick-borne intracellular hemoprotozoan parasite that is widespread across China. Genetic diversity is an important strategy used by parasites to escape the immune responses of their hosts. In our present study, 575 blood samples, collected from cattle in 10 provinces, were initially screened using a nested PCR (polymerase chain reaction) for detection of B. bovis infection. To perform genetic diversity analyses, positive samples were further amplified to obtain sequences of three B. bovis merozoite surface antigen genes (MSA-1, MSA-2b, MSA-2c). The results of the nested PCR approach showed that an average of 8.9% (51/575) of cattle were positive for B. bovis infection. Phylogenetic analyses of the predicted amino acid sequences revealed that unique antigen variants were formed only by Chinese isolates. Our findings provide vital information for understanding the genetic diversity of B. bovis in China. View Full-Text
Keywords: Babesia bovis; genetic diversity; merozoite surface antigen; China Babesia bovis; genetic diversity; merozoite surface antigen; China
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MDPI and ACS Style

Wang, J.; Yang, J.; Gao, S.; Wang, X.; Sun, H.; Lv, Z.; Li, Y.; Liu, A.; Liu, J.; Luo, J.; Guan, G.; Yin, H. Genetic Diversity of Babesia bovis MSA-1, MSA-2b and MSA-2c in China. Pathogens 2020, 9, 473.

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