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Paenibacillus alvei MP1 as a Producer of the Proteinaceous Compound with Activity against Important Human Pathogens, Including Staphylococcus aureus and Listeria monocytogenes

1
Department of Pharmaceutical Technology and Biochemistry, Faculty of Chemistry, Gdansk University of Technology, G. Narutowicza Street 11/12, 80-233 Gdansk, Poland
2
Department of Food Science, Cornell University, Stocking Hall, Ithaca, New York, NY 14853-7201, USA
*
Authors to whom correspondence should be addressed.
Part of the research was presented as a poster entitled “In search of antimicrobial compounds produced by Paenibacillus alvei isolated from buckwheat honey,” at International Conference on Biotechnology and Bioengineering (9th ICBB 2019); September 25–28, 2019 Poznan, Poland, co-organized by Asia-Pacific Association of Science, Engineering and Technology, Institute of Bioorganic Chemistry Polish Academy of Sciences, Adam Mickiewicz University, and Chinese Journal of Biologicals, in collaboration with multiple academic affiliations.
Pathogens 2020, 9(5), 319; https://doi.org/10.3390/pathogens9050319
Received: 1 April 2020 / Revised: 20 April 2020 / Accepted: 23 April 2020 / Published: 25 April 2020
An emerging need for new classes of antibiotics is, on the one hand, evident as antimicrobial resistance continues to rise. On the other hand, the awareness of the pros and cons of chemically synthesized compounds’ extensive use leads to a search for new metabolites in already known reservoirs. Previous research showed that Paenibacillus strain (P. alvei MP1) recovered from a buckwheat honey sample presented a wide spectrum of antimicrobial activity against both Gram-positive and Gram-negative pathogens. Recent investigation has confirmed that P. alvei MP1 (deposited at DDBJ/ENA/GenBank under the accession WSQB00000000) produces a proteinaceous, heat-stable compound(s) with the maximum antimicrobial production obtained after 18 h of P. alvei MP1 growth in LB medium at 37 °C with continuous shaking at 200 RPM. The highest activity was found in the 40% ammonium sulfate precipitate, with high activity also remaining in the 50% and 60% ammonium sulfate precipitates. Moderate to high antimicrobial activity that is insensitive to proteases or heat treatment, was confirmed against pathogenic bacteria that included L. monocytogenes FSL – X1-0001 (strain 10403S), S. aureus L1 – 0030 and E. coli O157: H7. Further studies, including de novo sequencing of peptides by mass spectrometry, are in progress. View Full-Text
Keywords: Paenibacillus alvei; antimicrobial activity; Staphylococcus aureus; Listeria monocytogenes; Escherichia coli; antimicrobial compounds; antimicrobial peptides Paenibacillus alvei; antimicrobial activity; Staphylococcus aureus; Listeria monocytogenes; Escherichia coli; antimicrobial compounds; antimicrobial peptides
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Pajor, M.; Xiong, Z.R.; Worobo, R.W.; Szweda, P. Paenibacillus alvei MP1 as a Producer of the Proteinaceous Compound with Activity against Important Human Pathogens, Including Staphylococcus aureus and Listeria monocytogenes. Pathogens 2020, 9, 319.

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