Next Article in Journal
In Silico Survey and Characterization of Babesia microti Functional and Non-Functional Proteases
Next Article in Special Issue
Campylocarpon fasciculare (Nectriaceae, Sordariomycetes); Novel Emergence of Black-Foot Causing Pathogen on Young Grapevines in China
Previous Article in Journal
Overexpression of Plasmodium falciparum M1 Aminopeptidase Promotes an Increase in Intracellular Proteolysis and Modifies the Asexual Erythrocytic Cycle Development
Previous Article in Special Issue
Fusarium elaeidis Causes Stem and Root Rot on Alocasia longiloba in South China
Article

“Shining a LAMP” (Loop-Mediated Isothermal Amplification) on the Molecular Detection of Phytopathogens Phytophthora spp. and Phytophthora cactorum in Strawberry Fields

Institute of Agrophysics, Polish Academy of Sciences, Doświadczalna 4, 20-290 Lublin, Poland
*
Author to whom correspondence should be addressed.
Academic Editor: Kevin D. Hyde
Pathogens 2021, 10(11), 1453; https://doi.org/10.3390/pathogens10111453
Received: 27 October 2021 / Revised: 7 November 2021 / Accepted: 9 November 2021 / Published: 10 November 2021
(This article belongs to the Special Issue Filamentous Fungal Pathogens)
Phytopathogenic microorganisms belonging to the genus Phytophthora have been recognized many times as causal agents of diseases that lower the yield of many plants important for agriculture. Meanwhile, Phytophthora cactorum causes crown rot and leather rot of berry fruits, mainly strawberries. However, widely-applied culture-based methods used for the detection of pathogens are time-consuming and often inaccurate. What is more, molecular techniques require costly equipment. Here we show a rapid and effective detection method for the aforementioned targets, deploying a simple molecular biology technique, Loop-Mediated Isothermal Amplification (LAMP). We optimized assays to amplify the translation elongation factor 1-α (EF1a) gene for two targets: Phytophthora spp. And Phytophthora cactorum. We optimized the LAMP on pure strains of the pathogens, isolated from organic plantations of strawberry, and successfully validated the assay on biological material from the environment including soil samples, rhizosphere, shoots and roots of strawberry, and with SYBR Green. Our results demonstrate that a simple and reliable molecular detection method, that requires only a thermoblock and simple DNA isolation kit, can be successfully applied to detect pathogens that are difficult to separate from the field. We anticipate our findings to be a starting point for developing easier and faster modifications of the isothermal detection methods and which can be applied directly in the plantation, in particular with the use of freeze-dried reagents and chemistry, allowing observation of the results with the naked eye. View Full-Text
Keywords: Loop-Mediated Isothermal Amplification; Phytophthora cactorum; Phytophthora spp.; rhizosphere; soil; shoots; roots; simple detection Loop-Mediated Isothermal Amplification; Phytophthora cactorum; Phytophthora spp.; rhizosphere; soil; shoots; roots; simple detection
Show Figures

Figure 1

MDPI and ACS Style

Siegieda, D.G.; Panek, J.; Frąc, M. “Shining a LAMP” (Loop-Mediated Isothermal Amplification) on the Molecular Detection of Phytopathogens Phytophthora spp. and Phytophthora cactorum in Strawberry Fields. Pathogens 2021, 10, 1453. https://doi.org/10.3390/pathogens10111453

AMA Style

Siegieda DG, Panek J, Frąc M. “Shining a LAMP” (Loop-Mediated Isothermal Amplification) on the Molecular Detection of Phytopathogens Phytophthora spp. and Phytophthora cactorum in Strawberry Fields. Pathogens. 2021; 10(11):1453. https://doi.org/10.3390/pathogens10111453

Chicago/Turabian Style

Siegieda, Dominika G., Jacek Panek, and Magdalena Frąc. 2021. "“Shining a LAMP” (Loop-Mediated Isothermal Amplification) on the Molecular Detection of Phytopathogens Phytophthora spp. and Phytophthora cactorum in Strawberry Fields" Pathogens 10, no. 11: 1453. https://doi.org/10.3390/pathogens10111453

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop