Quality Control and Mating Performance of Irradiated Glossina palpalis gambiensis Males
Abstract
:Simple Summary
Abstract
1. Introduction
2. Material and Methods
2.1. Tsetse Flies
2.2. Chilling and Irradiation of Male Pupae and Dosimetry of the Irradiator
2.3. Experiment 1: Quality Control of Irradiated Male Pupae
2.4. Experiment 2: Insemination Capacity of Irradiated Male G. p. gambiensis
2.5. Experiment 3: Female-Induced Sterility Levels when Mated with Irradiated Male Glossina palpalis gambiensis
2.6. Experiment 4: Male Mating Performance in a Walk-In Field Cage
2.7. Mating Indices and Data Analysis
3. Results
3.1. Experiment 1: Quality Control of Irradiated Male Pupae
3.2. Experiment 2: Sterile Male Insemination Capacity
3.3. Experiment 3: Female Induced Sterility Levels Mated with Irradiated Male Glossina palpalis gambiensis
3.4. Experiment 4: Male Mating Performance in Walk-In Field Cage
4. Discussion
5. Conclusions
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Treatment | Emergence Rate | Flight Ability | Non-Fliers Flies | Flies with Deformed Wings |
---|---|---|---|---|
Control | 94.55 ± 5.93 a | 89.58 ± 13.29 a | 7.83 ± 9.95 a | 2.59 ± 4.38 a |
Irradiated | 92.92 ± 5.79 a | 79.87 ± 12.60 b | 15.08 ± 10.42 b | 5.05 ± 3.57 b |
Treatment | No. Females Dissected | Insemination % | Spermathecae Fill (%) | |||
---|---|---|---|---|---|---|
0.25 | 0.5 | 0.75 | 1 | |||
Control | 368 | 100 | 18 (4.89) | 16 (4.35) | 35 (9.51) | 299 (81.25) |
Irradiated | 406 | 100 | 14 (3.45) | 27 (6.65) | 35 (8.62) | 330 (81.28) |
Treatment | No. Pupae Produced | No. Female X Days | Productivity per Female per Days | Sterility % |
---|---|---|---|---|
Control | 284 | 1821 | 0.1559 | 0 |
Irradiated | 26 | 1608 | 0.0161 | 89.7 |
Spermathecae Fill | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Treatments | Possible Pairs | Pairs | Mating Latency (Min ± SD) | Mating Duration (Min ± SD) | Propensity of Mating (PM) | Relative Mating Index (RMI ± SD) | 0 (0%) | 0.25 (%) | 0.5 (%) | 0.75 (%) | 1 (%) | Insemination Rate (%) | Relative Mating Performance (RMP) |
Control | 150 | 48 | 83.9 ± 48.83 | 45.48 ± 12.50 | 0.57 | 0.56 | 5 (10.42) | 4 (8.33) | 1 (2.08) | 7 (14.58) | 31 (64.58) | 89.58 | 0.07 |
Irradiated | - | 38 | 81.9 ± 54.79 | 45.16 ± 15.62 | - | 0.44 | 8 (21,05) | 2 (5.26) | 2 (5.26) | 0 | 26 (68.42) | 78.75 |
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Ilboudo, K.; Camara, K.; Salou, E.W.; Gimonneau, G. Quality Control and Mating Performance of Irradiated Glossina palpalis gambiensis Males. Insects 2022, 13, 476. https://doi.org/10.3390/insects13050476
Ilboudo K, Camara K, Salou EW, Gimonneau G. Quality Control and Mating Performance of Irradiated Glossina palpalis gambiensis Males. Insects. 2022; 13(5):476. https://doi.org/10.3390/insects13050476
Chicago/Turabian StyleIlboudo, Kadidiata, Karifa Camara, Ernest W. Salou, and Geoffrey Gimonneau. 2022. "Quality Control and Mating Performance of Irradiated Glossina palpalis gambiensis Males" Insects 13, no. 5: 476. https://doi.org/10.3390/insects13050476