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Open AccessArticle

Transcriptional Basis for Differential Thermosensitivity of Seedlings of Various Tomato Genotypes

1
Department of Biosciences, Molecular Cell Biology of Plants, Goethe University, D-60438 Frankfurt am Main, Germany
2
Buchmann Institute for Molecular Life Sciences, Goethe University, D-60438 Frankfurt am Main, Germany
3
Frankfurt Institute of Advanced Studies (FIAS), D-60438 Frankfurt am Main, Germany
4
Institute of Bioinformatics, University Medicine Greifswald, D-17475 Greifswald, Germany
*
Authors to whom correspondence should be addressed.
Genes 2020, 11(6), 655; https://doi.org/10.3390/genes11060655
Received: 19 May 2020 / Revised: 7 June 2020 / Accepted: 10 June 2020 / Published: 16 June 2020
(This article belongs to the Special Issue Heat Stress Response in Plants)
Transcriptional reprograming after the exposure of plants to elevated temperatures is a hallmark of stress response which is required for the manifestation of thermotolerance. Central transcription factors regulate the stress survival and recovery mechanisms and many of the core responses controlled by these factors are well described. In turn, pathways and specific genes contributing to variations in the thermotolerance capacity even among closely related plant genotypes are not well defined. A seedling-based assay was developed to directly compare the growth and transcriptome response to heat stress in four tomato genotypes with contrasting thermotolerance. The conserved and the genotype-specific alterations of mRNA abundance in response to heat stress were monitored after exposure to three different temperatures. The transcripts of the majority of genes behave similarly in all genotypes, including the majority of heat stress transcription factors and heat shock proteins, but also genes involved in photosynthesis and mitochondrial ATP production. In turn, genes involved in hormone and RNA-based regulation, such as auxin- and ethylene-related genes, or transcription factors like HsfA6b, show a differential regulation that associates with the thermotolerance pattern. Our results provide an inventory of genes likely involved in core and genotype-dependent heat stress response mechanisms with putative role in thermotolerance in tomato seedlings. View Full-Text
Keywords: heat stress; thermotolerance; Solanum lycopersicum L.; Massive Analysis of cDNA Ends (MACE); seedling; transcriptome; RNA-seq heat stress; thermotolerance; Solanum lycopersicum L.; Massive Analysis of cDNA Ends (MACE); seedling; transcriptome; RNA-seq
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Hu, Y.; Fragkostefanakis, S.; Schleiff, E.; Simm, S. Transcriptional Basis for Differential Thermosensitivity of Seedlings of Various Tomato Genotypes. Genes 2020, 11, 655.

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