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Article

Heterologous Expression of SvMBD5 from Salix viminalis L. Promotes Flowering in Arabidopsis thaliana L.

1
Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100193, China
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Beijing Academy of Forestry and Pomology Sciences, Beijing 100093, China
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Key Laboratory of Tree Breeding and Cultivation, State Forestry Administration, Beijing 100193, China
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College of Agriculture and Bioengineering, Heze University, Heze 274000, China
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School of Architectural and Artistic Design, Henan Polytechnic University, Jiaozuo 454000, China
*
Authors to whom correspondence should be addressed.
Genes 2020, 11(3), 285; https://doi.org/10.3390/genes11030285
Received: 7 February 2020 / Revised: 21 February 2020 / Accepted: 4 March 2020 / Published: 7 March 2020
(This article belongs to the Special Issue Epigenetic Mechanisms for Plant Gene Regulation)
Methyl-CpG-binding domain (MBD) proteins have diverse molecular and biological functions in plants. Most studies of MBD proteins in plants have focused on the model plant Arabidopsis thaliana L. Here we cloned SvMBD5 from the willow Salix viminalis L. by reverse transcription-polymerase chain reaction (RT-PCR) and analyzed the structure of SvMBD5 and its evolutionary relationships with proteins in other species. The coding sequence of SvMBD5 is 645 bp long, encoding a 214 amino acid protein with a methyl-CpG-binding domain. SvMBD5 belongs to the same subfamily as AtMBD5 and AtMBD6 from Arabidopsis. Subcellular localization analysis showed that SvMBD5 is only expressed in the nucleus. We transformed Arabidopsis plants with a 35S::SvMBD5 expression construct to examine SvMBD5 function. The Arabidopsis SvMBD5-expressing line flowered earlier than the wild type. In the transgenic plants, the expression of FLOWERING LOCUS T and CONSTANS significantly increased, while the expression of FLOWERING LOCUS C greatly decreased. In addition, heterologously expressing SvMBD5 in Arabidopsis significantly inhibited the establishment and maintenance of methylation of CHROMOMETHYLASE 3 and METHYLTRANSFERASE 1, as well as their expression, and significantly increased the expression of the demethylation-related genes REPRESSOR OF SILENCING1 and DEMETER-LIKE PROTEIN3. Our findings suggest that SvMBD5 participates in the flowering process by regulating the methylation levels of flowering genes, laying the foundation for further studying the role of SvMBD5 in regulating DNA demethylation. View Full-Text
Keywords: Salix viminalis; SvMBD5; transgenic; flowering; demethylation Salix viminalis; SvMBD5; transgenic; flowering; demethylation
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MDPI and ACS Style

Cheng, Y.; Cheng, L.; Cao, Q.; Zou, J.; Li, X.; Ma, X.; Zhou, J.; Zhai, F.; Sun, Z.; Lan, Y.; Han, L. Heterologous Expression of SvMBD5 from Salix viminalis L. Promotes Flowering in Arabidopsis thaliana L. Genes 2020, 11, 285. https://doi.org/10.3390/genes11030285

AMA Style

Cheng Y, Cheng L, Cao Q, Zou J, Li X, Ma X, Zhou J, Zhai F, Sun Z, Lan Y, Han L. Heterologous Expression of SvMBD5 from Salix viminalis L. Promotes Flowering in Arabidopsis thaliana L. Genes. 2020; 11(3):285. https://doi.org/10.3390/genes11030285

Chicago/Turabian Style

Cheng, Yunhe, Lili Cheng, Qingchang Cao, Junzhu Zou, Xia Li, Xiaodong Ma, Jingjing Zhou, Feifei Zhai, Zhenyuan Sun, Yanping Lan, and Lei Han. 2020. "Heterologous Expression of SvMBD5 from Salix viminalis L. Promotes Flowering in Arabidopsis thaliana L." Genes 11, no. 3: 285. https://doi.org/10.3390/genes11030285

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