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Open AccessArticle

Genomic Diversity of Two Hydrocarbon-Degrading and Plant Growth-Promoting Pseudomonas Species Isolated from the Oil Field of Bóbrka (Poland)

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Environmental Biology, Centre for Environmental Sciences, Hasselt University, Agoralaan Building D, 3590 Diepenbeek, Belgium
2
Centre of Excellence PLECO, Department of Biology, University of Antwerp, Universiteitsplein 1, B-2610 Wilrijk, Belgium
3
Department of Biology Sciences, Thompson Rivers University, 805 TRU Way, Kamloops, BC V2C 0C8, Canada
4
Warsaw University of Life Sciences, Faculty of Horticulture, Biotechnology and Landscape Architecture, Nowoursynowska Road 159, 02-776 Warsaw, Poland
5
Department of Plant Physiology, Faculty of Biology and Biotechnology, Maria Skłodowska-Curie University, 20-950 Lublin, Poland
*
Author to whom correspondence should be addressed.
Genes 2019, 10(6), 443; https://doi.org/10.3390/genes10060443
Received: 13 April 2019 / Revised: 3 June 2019 / Accepted: 4 June 2019 / Published: 11 June 2019
(This article belongs to the Special Issue Genetics of Biodegradation and Bioremediation)
Hydrocarbon-degrading bacteria are important resources for use in phytoremediation applications. Yet, for many hydrocarbonoclastic strains the genetic information regarding pollutant degradation and detoxification has not been thoroughly revealed. In this study, hydrocarbon-degrading bacteria were isolated from a long-term oil-polluted soil in Bóbrka, Poland. Pseudomonas spp. was the most dominant species. Of all 69 isolated strains tested in the laboratory using qualitative biochemical assays, 61% showed the capability to use diesel as sole carbon source, 33% could produce indole, 19% produced siderophores, 36% produced organic acids, and 54% were capable of producing 1-aminocyclopropane-1-carboxylate (ACC)-deaminase. From all morphologically and genetically different strains, two representative Pseudomonas spp., strain VI4.1 and VI4T1, were selected for genome sequencing. Genomic analyses indicated the presence of the full naphthalene dioxygenase operon (plasmid and chromosomal), of genes involved in the degradation of BTEX compounds (Benzene, Toluene, Ethylbenzene, Xylene) and alkanes (alkB gene) as well as the anthranilate degradation pathway (strain VI4T1) and terephthalate dioxygenase protein (strain VI4.1). Proton transfer reaction time-of-flight mass spectrometry (PTR-TOF-MS) analyses confirmed naphthalene and BTEX degradation within seven days. Motility, resistance to abiotic stresses, high and low temperatures, low pH, and salinity were confirmed at the genetic level and experimentally verified. The presence of multiple degradative and plant growth promotion genes, together with the in vitro experimental evidence, indicates the high value of these two strains and their potential use for sustainable site clean-up. View Full-Text
Keywords: naphthalene; toluene; hydrocarbons; plant growth promotion; bioremediation; Pseudomonas; soil pollution; phytoremediation naphthalene; toluene; hydrocarbons; plant growth promotion; bioremediation; Pseudomonas; soil pollution; phytoremediation
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Imperato, V.; Portillo-Estrada, M.; McAmmond, B.M.; Douwen, Y.; Van Hamme, J.D.; Gawronski, S.W.; Vangronsveld, J.; Thijs, S. Genomic Diversity of Two Hydrocarbon-Degrading and Plant Growth-Promoting Pseudomonas Species Isolated from the Oil Field of Bóbrka (Poland). Genes 2019, 10, 443.

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