Next Article in Journal
Identification of Fatty Acid Desaturases in Maize and Their Differential Responses to Low and High Temperature
Next Article in Special Issue
DNA Methylation Module Network-Based Prognosis and Molecular Typing of Cancer
Previous Article in Journal
Genomic Diversity of Two Hydrocarbon-Degrading and Plant Growth-Promoting Pseudomonas Species Isolated from the Oil Field of Bóbrka (Poland)
Open AccessArticle

Methylome-Wide Association Study in Peripheral White Blood Cells Focusing on Central Obesity and Inflammation

Department of Nutrition, Food Sciences and Physiology, University of Navarra, Irunlarrea 1,31008 Pamplona, Spain
Centre for Nutrition Research, University of Navarra, Irunlarrea 1, 31008, Pamplona, Spain
Centro de Investigación Biomédica en Red Fisiopatología de la Obesidad y Nutrición (CIBERobn), Instituto de Salud Carlos III, 28029, Madrid, Spain
Navarra Institute for Health Research (IdiSNa), 31008, Pamplona, Spain
Department of Bioinformatics, Making Genetics S.L., 31002, Pamplona, Spain
Precision Nutrition and Cardiometabolic Health Program, Madrid Institute for Advanced Studies (IMDEA), IMDEA Food, 28049, Madrid, Spain
Author to whom correspondence should be addressed.
Genes 2019, 10(6), 444;
Received: 17 April 2019 / Revised: 3 June 2019 / Accepted: 7 June 2019 / Published: 11 June 2019
(This article belongs to the Special Issue DNA Methylation in Health and Diseases)
Epigenetic signatures such as DNA methylation may be associated with specific obesity traits in different tissues. The onset and development of some obesity-related complications are often linked to visceral fat accumulation. The aim of this study was to explore DNA methylation levels in peripheral white blood cells to identify epigenetic methylation marks associated with waist circumference (WC). DNA methylation levels were assessed using Infinium Human Methylation 450K and MethylationEPIC beadchip (Illumina) to search for putative associations with WC values of 473 participants from the Methyl Epigenome Network Association (MENA) project. Statistical analysis and Ingenuity Pathway Analysis (IPA) were employed for assessing the relationship between methylation and WC. A total of 669 CpGs were statistically associated with WC (FDR < 0.05, slope ≥ |0.1|). From these CpGs, 375 CpGs evidenced a differential methylation pattern between females with WC ≤ 88 and > 88 cm, and 95 CpGs between males with WC ≤ 102 and > 102 cm. These differentially methylated CpGs are located in genes related to inflammation and obesity according to IPA. Receiver operating characteristic (ROC) curves of the top four significant differentially methylated CpGs separated by sex discriminated individuals with presence or absence of abdominal fat. ROC curves of all the CpGs from females and one CpG from males were validated in an independent sample (n = 161). These methylation results add further insights about the relationships between obesity, adiposity-associated comorbidities, and DNA methylation where inflammation processes may be involved. View Full-Text
Keywords: waist circumference; epigenetics; DNA methylation waist circumference; epigenetics; DNA methylation
Show Figures

Figure 1

MDPI and ACS Style

Arpón, A.; Milagro, F.I.; Ramos-Lopez, O.; Mansego, M.L.; Riezu-Boj, J.-I.; Martínez, J.A., on Behalf of the MENA Project; Methylome-Wide Association Study in Peripheral White Blood Cells Focusing on Central Obesity and Inflammation. Genes 2019, 10, 444.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

Back to TopTop