Next Article in Journal
Characterization of Two Novel Intronic Variants Affecting Splicing in FBN1-Related Disorders
Previous Article in Journal
Association between Aerobic Exercise and High-Density Lipoprotein Cholesterol Levels across Various Ranges of Body Mass Index and Waist-Hip Ratio and the Modulating Role of the Hepatic Lipase rs1800588 Variant
Open AccessArticle

A Wheat β-Patchoulene Synthase Confers Resistance against Herbivory in Transgenic Arabidopsis

Institute of Ecological Agriculture, College of Agronomy, Sichuan Agricultural University, Chengdu 611130, China
*
Author to whom correspondence should be addressed.
These authors contributed equally.
Genes 2019, 10(6), 441; https://doi.org/10.3390/genes10060441
Received: 5 May 2019 / Revised: 27 May 2019 / Accepted: 4 June 2019 / Published: 10 June 2019
(This article belongs to the Section Plant Genetics and Genomics)
Terpenoids play important roles in plant defense. Although some terpene synthases have been characterized, terpenoids and their biosynthesis in wheat (Triticum aestivum L.) still remain largely unknown. Here, we describe the identification of a terpene synthase gene in wheat. It encodes a sesquiterpene synthase that catalyzes β-patchoulene formation with E,E-farnesyl diphosphate (FPP) as the substrate, thus named as TaPS. TaPS exhibits inducible expression in wheat in response to various elicitations. Particularly, alamethicin treatment strongly induces TaPS gene expression and β-patchoulene accumulation in wheat. Overexpression of TaPS in Arabidopsis successfully produces β-patchoulene, verifying the biochemical function of TaPS in planta. Furthermore, these transgenic Arabidopsis plants exhibit resistance against herbivory by repelling beet armyworm larvae feeding, thereby indicating anti-herbivory activity of β-patchoulene. The catalytic mechanism of TaPS is also explored by homology modeling and site-directed mutagenesis. Two key amino acids are identified to act in protonation and stability of intermediates and product formation. Taken together, one wheat sesquiterpene synthase is identified as β-patchoulene synthase. TaPS exhibits inducible gene expression and the sesquiterpene β-patchoulene is involved in repelling insect infestation. View Full-Text
Keywords: arabidopsis; herbivory; patchoulene; resistance; sesquiterpene; wheat arabidopsis; herbivory; patchoulene; resistance; sesquiterpene; wheat
Show Figures

Figure 1

MDPI and ACS Style

Pu, Q.; Liang, J.; Shen, Q.; Fu, J.; Pu, Z.; Liu, J.; Wang, X.; Wang, Q. A Wheat β-Patchoulene Synthase Confers Resistance against Herbivory in Transgenic Arabidopsis. Genes 2019, 10, 441.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop