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Open AccessArticle

Functional Research on Three Presumed Asparagine Synthetase Family Members in Poplar

1
State Key Laboratory of Tree Genetics and Breeding (Northeast Forestry University), School of Forestry, Northeast Forestry University, Harbin 150040, China
2
School of Forestry, Northeast Forestry University, Harbin 150040, China
3
Guangxi Forestry Research Institute, Nanning 530000, China
4
College of Life Science, Northeast Forestry University, Harbin 150040, China
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Genes 2019, 10(5), 326; https://doi.org/10.3390/genes10050326
Received: 11 March 2019 / Revised: 16 April 2019 / Accepted: 23 April 2019 / Published: 28 April 2019
(This article belongs to the Section Plant Genetics and Genomics)
Asparagine synthetase (AS), a key enzyme in plant nitrogen metabolism, plays an important role in plant nitrogen assimilation and distribution. Asparagine (Asn), the product of asparagine synthetase, is one of the main compounds responsible for organic nitrogen transport and storage in plants. In this study, we performed complementation experiments using an Asn-deficient Escherichia coli strain to demonstrate that three putative asparagine synthetase family members in poplar (Populus simonii × P. nigra) function in Asn synthesis. Quantitative real-time PCR revealed that the three members had high expression levels in different tissues of poplar and were regulated by exogenous nitrogen. PnAS1 and PnAS2 were also affected by diurnal rhythm. Long-term dark treatment resulted in a significant increase in PnAS1 and PnAS3 expression levels. Under long-term light conditions, however, PnAS2 expression decreased significantly in the intermediate region of leaves. Exogenous application of ammonium nitrogen, glutamine, and a glutamine synthetase inhibitor revealed that PnAS3 was more sensitive to exogenous glutamine, while PnAS1 and PnAS2 were more susceptible to exogenous ammonium nitrogen. Our results suggest that the various members of the PnAS gene family have distinct roles in different tissues and are regulated in different ways. View Full-Text
Keywords: poplar; asparagine synthetase; functional research; expression patterns; transcriptional regulation poplar; asparagine synthetase; functional research; expression patterns; transcriptional regulation
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MDPI and ACS Style

Qu, C.; Hao, B.; Xu, X.; Wang, Y.; Yang, C.; Xu, Z.; Liu, G. Functional Research on Three Presumed Asparagine Synthetase Family Members in Poplar. Genes 2019, 10, 326.

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