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Open AccessArticle

Identification of Genes Involved in Low Temperature-Induced Senescence of Panicle Leaf in Litchi chinensis

1
Guangdong Litchi Engineering Research Center, College of Horticulture, South China Agricultural University, Guangzhou 510642, China
2
Guangdong Academy of Forestry, Guangzhou 510520, China
3
State Key Laboratory of Agrobiotechnology, School of Life Sciences, Chinese University of Hong Kong, Hong Kong, China
4
Key Laboratory of Tropic Fruit Trees, Hainan Academy of Agricultural Science, Haikou 571100, China
*
Authors to whom correspondence should be addressed.
These authors have contributed equally to this work.
Genes 2019, 10(2), 111; https://doi.org/10.3390/genes10020111
Received: 10 January 2019 / Revised: 25 January 2019 / Accepted: 28 January 2019 / Published: 1 February 2019
(This article belongs to the Special Issue Genetics of Plant Senescence)
Warm winters and hot springs may promote panicle leaf growing and repress floral development. To identify genes potentially involved in litchi panicle leaf senescence, eight RNA-sequencing (RNA-Seq) libraries of the senescing panicle leaves under low temperature (LT) conditions and the developing panicle leaves under high temperature (HT) conditions were constructed. For each library, 4.78–8.99 × 106 clean reads were generated. Digital expression of the genes was compared between the senescing and developing panicle leaves. A total of 6477 upregulated differentially expressed genes (DEGs) (from developing leaves to senescing leaves), and 6318 downregulated DEGs were identified, 158 abscisic acid (ABA)-, 68 ethylene-, 107 indole-3-acetic acid (IAA)-, 27 gibberellic acid (GA)-, 68 cytokinin (CTK)-, 37 salicylic acid (SA)-, and 23 brassinolide (BR)-related DEGs. Confirmation of the RNA-Seq data by quantitative real-time PCR (qRT-PCR) analysis suggested that expression trends of the 10 candidate genes using qRT-PCR were similar to those revealed by RNA-Seq, and a significantly positive correlation between the obtained data from qRT-PCR and RNA-Seq were found, indicating the reliability of our RNA-Seq data. The present studies provided potential genes for the future molecular breeding of new cultivars that can induce panicle leaf senescence and reduce floral abortion under warm climates. View Full-Text
Keywords: panicle leaf; senescence; transcriptome; litchi; low temperature panicle leaf; senescence; transcriptome; litchi; low temperature
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MDPI and ACS Style

Wang, C.; Liu, H.; Yu, S.; Chen, H.; Hu, F.; Zhan, H.; Pan, X.; Lao, Y.; Zhong, S.; Zhou, B. Identification of Genes Involved in Low Temperature-Induced Senescence of Panicle Leaf in Litchi chinensis. Genes 2019, 10, 111. https://doi.org/10.3390/genes10020111

AMA Style

Wang C, Liu H, Yu S, Chen H, Hu F, Zhan H, Pan X, Lao Y, Zhong S, Zhou B. Identification of Genes Involved in Low Temperature-Induced Senescence of Panicle Leaf in Litchi chinensis. Genes. 2019; 10(2):111. https://doi.org/10.3390/genes10020111

Chicago/Turabian Style

Wang, Congcong; Liu, Hao; Yu, Sheng; Chen, Houbin; Hu, Fuchu; Zhan, Huiling; Pan, Xifen; Lao, Yuhua; Zhong, Silin; Zhou, Biyan. 2019. "Identification of Genes Involved in Low Temperature-Induced Senescence of Panicle Leaf in Litchi chinensis" Genes 10, no. 2: 111. https://doi.org/10.3390/genes10020111

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