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Article

Cellular Response to Vitamin C-Enriched Chitosan/Agarose Film with Potential Application as Artificial Skin Substitute for Chronic Wound Treatment

Department of Biochemistry and Biotechnology, Medical University of Lublin, Chodzki 1 Street, 20-093 Lublin, Poland
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Author to whom correspondence should be addressed.
Cells 2020, 9(5), 1185; https://doi.org/10.3390/cells9051185
Received: 14 April 2020 / Revised: 30 April 2020 / Accepted: 8 May 2020 / Published: 10 May 2020
(This article belongs to the Special Issue Stem Cell Therapy: Wound Healing and Skin Regeneration)
The treatment of chronic wounds is still a meaningful challenge to physicians. The aim of this work was to produce vitamin C-enriched chitosan/agarose (CHN/A) film that could serve as potential artificial skin substitute for chronic wound treatment. The biomaterial was fabricated by a newly developed and simplified method via mixing acidic chitosan solution with alkaline agarose solution that allowed to obtain slightly acidic pH (5.97) of the resultant material, which is known to support skin regeneration. Vitamin C was immobilized within the matrix of the film by entrapment method during production process. Produced films (CHN/A and CHN/A + vit C) were subjected to comprehensive evaluation of cellular response with the use of human skin fibroblasts, epidermal keratinocytes, and macrophages. It was demonstrated that novel biomaterials support adhesion and growth of human skin fibroblasts and keratinocytes, have ability to slightly reduce transforming growth factor-beta 1 (TGF-β1) (known to be present at augmented levels in the epidermis of chronic wounds), and increase platelet-derived growth factor-BB (PDGF-BB) secretion by the cells. Nevertheless, addition of vitamin C to the biomaterial formulation does not significantly improve its biological properties due to burst vitamin release profile. Obtained results clearly demonstrated that produced CHN/A film has great potential to be used as cellular dermal, epidermal, or dermo-epidermal graft pre-seeded with human skin cells for chronic wound treatment. View Full-Text
Keywords: biocompatibility; cell migration; proliferation; fibroblasts; keratinocytes; dermal skin grafts; epidermal skin grafts; drug release profile biocompatibility; cell migration; proliferation; fibroblasts; keratinocytes; dermal skin grafts; epidermal skin grafts; drug release profile
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MDPI and ACS Style

Vivcharenko, V.; Wojcik, M.; Przekora, A. Cellular Response to Vitamin C-Enriched Chitosan/Agarose Film with Potential Application as Artificial Skin Substitute for Chronic Wound Treatment. Cells 2020, 9, 1185. https://doi.org/10.3390/cells9051185

AMA Style

Vivcharenko V, Wojcik M, Przekora A. Cellular Response to Vitamin C-Enriched Chitosan/Agarose Film with Potential Application as Artificial Skin Substitute for Chronic Wound Treatment. Cells. 2020; 9(5):1185. https://doi.org/10.3390/cells9051185

Chicago/Turabian Style

Vivcharenko, Vladyslav, Michal Wojcik, and Agata Przekora. 2020. "Cellular Response to Vitamin C-Enriched Chitosan/Agarose Film with Potential Application as Artificial Skin Substitute for Chronic Wound Treatment" Cells 9, no. 5: 1185. https://doi.org/10.3390/cells9051185

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