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Open AccessArticle

Involvement of TRPC4 and 5 Channels in Persistent Firing in Hippocampal CA1 Pyramidal Cells

by Alberto Arboit 1,2,3, Antonio Reboreda 1,4 and Motoharu Yoshida 1,3,4,5,*
1
German Center for Neurodegenerative Diseases (DZNE), 39120 Magdeburg, Germany
2
Otto-von-Guericke University, 39120 Magdeburg, Germany
3
Faculty of Psychology, Ruhr University Bochum (RUB), Universitätsstraße 150, 44801 Bochum, Germany
4
Leibniz Institute for Neurobiology (LIN), 39118 Magdeburg, Germany
5
Center for Behavioral Brain Sciences (CBBS), 39106 Magdeburg, Germany
*
Author to whom correspondence should be addressed.
Cells 2020, 9(2), 365; https://doi.org/10.3390/cells9020365
Received: 1 December 2019 / Revised: 29 January 2020 / Accepted: 1 February 2020 / Published: 5 February 2020
(This article belongs to the Special Issue TRPC Channels)
Persistent neural activity has been observed in vivo during working memory tasks, and supports short-term (up to tens of seconds) retention of information. While synaptic and intrinsic cellular mechanisms of persistent firing have been proposed, underlying cellular mechanisms are not yet fully understood. In vitro experiments have shown that individual neurons in the hippocampus and other working memory related areas support persistent firing through intrinsic cellular mechanisms that involve the transient receptor potential canonical (TRPC) channels. Recent behavioral studies demonstrating the involvement of TRPC channels on working memory make the hypothesis that TRPC driven persistent firing supports working memory a very attractive one. However, this view has been challenged by recent findings that persistent firing in vitro is unchanged in TRPC knock out (KO) mice. To assess the involvement of TRPC channels further, we tested novel and highly specific TRPC channel blockers in cholinergically induced persistent firing in mice CA1 pyramidal cells for the first time. The application of the TRPC4 blocker ML204, TRPC5 blocker clemizole hydrochloride, and TRPC4 and 5 blocker Pico145, all significantly inhibited persistent firing. In addition, intracellular application of TRPC4 and TRPC5 antibodies significantly reduced persistent firing. Taken together these results indicate that TRPC4 and 5 channels support persistent firing in CA1 pyramidal neurons. Finally, we discuss possible scenarios causing these controversial observations on the role of TRPC channels in persistent firing. View Full-Text
Keywords: TRPC channels; intrinsic persistent activity; cholinergic modulation; patch clamp; hippocampus; TRPC antagonists; working memory TRPC channels; intrinsic persistent activity; cholinergic modulation; patch clamp; hippocampus; TRPC antagonists; working memory
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Arboit, A.; Reboreda, A.; Yoshida, M. Involvement of TRPC4 and 5 Channels in Persistent Firing in Hippocampal CA1 Pyramidal Cells. Cells 2020, 9, 365.

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