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Open AccessArticle

Omics Studies Revealed the Factors Involved in the Formation of Colony Boundary in Myxococcus xanthus

1
State Key Laboratory of Microbial Technology, Institute of Microbial Technology, Shandong University, Qingdao 266237, China
2
Department of Research and Development, Uttaranchal University, Dehradun 248007, India
*
Author to whom correspondence should be addressed.
Cells 2019, 8(6), 530; https://doi.org/10.3390/cells8060530
Received: 10 April 2019 / Revised: 29 May 2019 / Accepted: 31 May 2019 / Published: 3 June 2019
Two unrecognizable strains of the same bacterial species form a distinct colony boundary. During growth as colonies, Myxococcus xanthus uses multiple factors to establish cooperation between recognized strains and prevent interactions with unrecognized strains of the same species. Here, ΔMXAN_0049 is a mutant strain deficient in immunity for the paired nuclease gene, MXAN_0050, that has a function in the colony-merger incompatibility of Myxococcus xanthus DK1622. With the aim to investigate the factors involved in boundary formation, a proteome and metabolome study was employed. Visualization of the boundary between DK1622 and ΔMXAN_0049 was done scanning electron microscope (SEM), which displayed the presence of many damaged cells in the boundary. Proteome analysis of the DK1622- boundary disclosed many possible proteins, such as cold shock proteins, cell shape-determining protein MreC, along with a few pathways, such as RNA degradation, phenylalanine, tyrosine and tryptophan biosynthesis, and Type VI secretion system (T6SS), which may play major roles in the boundary formation. Metabolomics studies revealed various secondary metabolites that were significantly produced during boundary formation. Overall, the results concluded that multiple factors participated in the boundary formation in M. xanthus, leading to cellular damage that is helpful in solving the mystery of the boundary formation mechanism. View Full-Text
Keywords: type VI secretion system; proteomics; Myxococcus xanthus; secondary metabolites; microscopy; cold shock proteins type VI secretion system; proteomics; Myxococcus xanthus; secondary metabolites; microscopy; cold shock proteins
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Anwar, M.N.; Li, Z.F.; Gong, Y.; Singh, R.P.; Li, Y.-Z. Omics Studies Revealed the Factors Involved in the Formation of Colony Boundary in Myxococcus xanthus. Cells 2019, 8, 530.

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