Next Article in Journal
Niclosamide Triggers Non-Canonical LC3 Lipidation
Previous Article in Journal
The Emerging Roles of TERRA in Telomere Maintenance and Genome Stability
Open AccessArticle

Chronological Aging Standard Curves of Telomere Length and Mitochondrial DNA Copy Number in Twelve Tissues of C57BL/6 Male Mouse

1
Department of Anatomy and Convergence Medical Sciences, Gyeongsang National University School of Medicine, Jinju 52727, Korea
2
Institute of Health Sciences, Bio Anti-aging Medical Research Center, Gyeongsang National University, Jinju 52727, Korea
3
Department of Internal Medicine, Gyeongsang National University School of Medicine and Cardiovascular Center, Gyeongsang National University Changwon Hospital, Changwon 51472, Korea
4
Department of Pharmacology and Convergence Medical Sciences, Gyeongsang National University School of Medicine, Jinju 52727, Korea
*
Author to whom correspondence should be addressed.
Cells 2019, 8(3), 247; https://doi.org/10.3390/cells8030247
Received: 15 February 2019 / Revised: 11 March 2019 / Accepted: 12 March 2019 / Published: 15 March 2019
The changes in telomere length and mitochondrial DNA copy number (mtDNAcn) are considered to be aging markers. However, many studies have provided contradictory or only fragmentary information about changes of these markers in animal models, due to inaccurate analysis methods and a lack of objective aging standards. To establish chronological aging standards for these two markers, we analyzed telomere length and mtDNAcn in 12 tissues—leukocytes, prefrontal cortex, hippocampus, pituitary gland, adrenal gland, retina, aorta, liver, kidney, spleen, skeletal muscle, and skin—from a commonly used rodent model, C57BL/6 male mice aged 2–24 months. It was found that at least one of the markers changed age-dependently in all tissues. In the leukocytes, hippocampus, retina, and skeletal muscle, both markers changed age-dependently. As a practical application, the aging marker changes were analyzed after chronic immobilization stress (CIS) to see whether CIS accelerated aging or not. The degree of tissue-aging was calculated using each standard curve and found that CIS accelerated aging in a tissue-specific manner. Therefore, it is expected that researchers can use our standard curves to objectively estimate tissue-specific aging accelerating effects of experimental conditions for least 12 tissues in C57BL/6 male mice. View Full-Text
Keywords: telomere length; mitochondrial DNA copy number; aging; chronic stress; mouse telomere length; mitochondrial DNA copy number; aging; chronic stress; mouse
Show Figures

Figure 1

MDPI and ACS Style

Baek, J.H.; Son, H.; Jeong, Y.-H.; Park, S.W.; Kim, H.J. Chronological Aging Standard Curves of Telomere Length and Mitochondrial DNA Copy Number in Twelve Tissues of C57BL/6 Male Mouse. Cells 2019, 8, 247.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop